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Revision as of 21:38, 12 February 2011

Welcome to the LEAP Technologies wiki, a digital brochure and literature catalog library of LEAP's PAL applications.

 ↳ leaptec.com
 ↳ leapwiki.com

Company Description

LEAP Technologies, Inc. was founded in 1989 as an autosampler company serving the GC and GC–MS markets. Since then, LEAP has expanded into front-end sample handling for all areas of chromatography and mass spectroscopy, including LC/MS/MS and MALDI-MS as well as robotics for proteomics and fiber optic UV technology for tablet dissolution. LEAP strives to find total solutions for analytical lab automation. One of the major components in LEAP's approach to automation solutions is with the PAL X,Y,Z robot.LEAP Technologies All Things PAL™ Simplify with One Call, One Source, One Purchase Order! Buy direct from LEAP & enjoy our undivided attention, product expertise, and more.

Visit LEAP at Lab Automation 2011 - Booth # 22
At LabAutomation 2011 LEAP with be premiering a fast, super accurate Micro Dispenser for 96-, 384-, 1536-, 3456- plate work; up to 5 channels with individual feeds for enormous flexibility

• PAL sampling device customization and multiplexing software.
  
• PAL Accessories offering diverse applications including semi-solid metering, gravimetric determinations with balances and solid phase extractions.
  
• LEAP Automated Dried Blood Spot Extraction(DBS) robot elutes blood directly into LC
  

The LEAP Story

LEAP is experienced in providing automation solutions for any process that gathers, treats and delivers samples to an analytical process. Liquid Handling is in most cases involved but we are not limited to that. We can also move,weigh and image objects. Liquid handling includes spraying and printing besides the usual measuring, pipetting, injecting, mixing , heating or sampling of a variety of liquids. We have the ability to design, engineer, deliver and support simple but unique or complex but simple to use systems. The LEAP Shell software lets us create solution-specific sample lists and shell methods that synchronize all instruments involved. LEAP Shell is used for LC/LCMS and stand-alone workstations. LEAP uses Chronos Mastersoftwarefor GC and GC-MS integrations. Currently, Chronos integrates with Thermo Electron Xcalibur 1.4 and 2.0 GC & Agilent GC and MSD ChemStation. Further data system packages are begin integrated with Chronos so check with LEAP to see if yours is.
We can provide complete locked-down systems to Value Added Resellers (VARs). Combinations of instruments that we can supply are robotic sampling devices and LC pumps as front-end to LC-MS systems. Single and dual channel LC to one MS that save cycle time and therefore increase throughput are becoming standard Frontend Automation Bundles (FABs). LEAP has available a proven SDK for PAL integration into your data system software.
One of LEAP's fundamental business philosophies is to build strong client relationships by knowing our customers and responding to their specific needs with superior personal service whether you have one PAL or are VAR of LEAP's.

Chief Chromatographic Techniques Supported:

• LC-Fraction Collection for purification(semi-prep)
• LC–MS Sample Handlers providing sample just before injection
• GC and GC–MS Sample Prep such as Headspace,SPME, 96-well Filtration, ITSP,MEPS, Derivatization, SPEwith different injection techniques
• LC pumps for 50nl-2ml flow rates applicable to standard, micro and capillary columns
• 2D LC systems with sample handlers PAL for nano spray, nano fractionation to MALDI and other fraction collection schemes

A Sampling of Markets Served:

• Pharma / Biotech
• Polymer / Chemical
• Proteomics
• Forensic
• Food and Beverage
• Fragrance
• Environmental
• Fuel

Application Solutions

Application Solutions

LEAP strives to find standardized solutions creating the total package for all industries using analytical processes for small and large molecules in extracted liquids, solids, and in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as mass spectroscopy. For more details, contact the LEAP office.

• Analyzing a Plant Extract

• Balance PAL

• Biofuel Applications

• Certus Liquid Dispenser

• Direct Analysis in Real Time (DART®)

• GC Headspace

• Filtering of Samples

• Hydrogen Deuterium Exchange

• LC Multi-Valve Applications

• MALDI Applications

• NMR Sample Prep

• Polymer Analysis

• Process Monitoring Applications

• Purification Applications

• Radiolabeling

• Refractometer

• Semi Solid Applications

• Small Volume Fraction Collection

• Solid Phase Extraction

• SPME

• Tobacco Analysis

LEAP Integrated Components

Accessories
PAL Accessories and Options - a concept you can grow with for your CTC Analytics Autosampler. For more details, contact the LEAP office.

• Bar Code Reading

• Column Heating

• Cooling and Heating Sample Storage

• Filtering of Samples

• Headspace

• Large Volume Dispensing

• Mixing of Samples

• Unique Storage

• Unique Injections

• Valve Washing

Examples of Special Solutions

Example Solutions

Examples of some customized applications we have provided. We call them "LEAP Specials". They are custom made to suit your exact needs. Let us show you how automation can make your life in the laboratory easier, more efficient, and more rewarding. Turn Your CTC Autosampler into a LEAP WorkStation. The CTC PAL is a great autosampler but LEAP can improve upon it with one or more of our automated solutions. You dont have to know how - you just have to know LEAP. LEAP is experienced in providing automation solutions for any process that gathers, treats and delivers samples to an analytical process. Liquid Handling is in most cases involved but we are not limited to that. We can also move, weigh and image objects. Liquid handling includes spraying and printing besides the usual measuring, pipetting, injecting, mixing, heating or sampling of a variety of liquids. We have the ability to design, engineer, deliver and support simple but unique or complex but simple to use systems. We have various software packages to choose from including all major data systems. Our own LEAP Shell scheduling software let’s us create solution specific sample lists and Shell methods that synchronizes all instruments involved. We also can use Chronos Mastersoftware, Cycle Composer software, Cycle Editor and ICC-CE depending on your needs.
For more details, contact the LEAP office and provide the project code.

• Analysis of Total Iodine

• A Flexible LCMS Front End for High-Throughput Bioanalytical Analysis in GLP Environment

• Automated online liquid chromatographic/mass spectrometric metabolic study for prodrug stability

• Detecting a Carcinogen in Alcohol Beverages

• Dilute and incubate sample prior to LC injection

• Dual Channel Sample Cleanup

• EPA Method 8272

• Forced Degradation

• Forced Degradation - Dual PAL under LEAP Shell

• High SPEed for CTC PAL On-line Multidimensional SPE-SPE-LC of Raw Biofluids

• High Throughput Sample Analysis using Multiple Column LC/MS/MS and Automated Method Optimization Techniques

• Intrinsic Viscosity testing

• Increasing throughput of parallel on-line extraction liquid chromatography/electrospray ionization tandem mass spectrometry system for GLP quantitative bioanalysis in drug development

• Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery

• Kinetic Reaction with GC Injection

• Measuring nicotine partitioning from a tobacco smoke filter pad in an ammonia environment

• Multiplexing LC injections

• Multi-Valve system for selectable loop size

• One Step Derivitization

• Online Sample Cleanup using column switching with Multi-Valve system

• On-line SPE LC/MS/MS to Detect Organonitrogen and Triazine Pesticides in Drinking Water

• Pooling of vial contents for QC testing

• Productivity Package

• Rapid Precision Dispensing

• Sample Filtration with LEAP X-Press

• Sandwich Technique for Small Sample Volumes

• Sharper peaks using 2-stage low sample dispersal injection technique

• Simultaneous Screen of 23 Drugs of Abuse by LC API MS MS

• Static Headspace Gas Chromatography – Mass Spectrometry(SHS-GC–MS) Method for Monitoring the Formation of ethyl methane sulfonate from ethanol and methane sulfonic acid

• Tobacco Filtration - 96 Well Plate Filtration

• Tobacco Filtration - Large Volume Flask

• Twin PAL - High Throughput injections using Cycle Composer

• Using the PAL Autosampler as a Syringe Pump for MS Tuning

Search LEAP Wiki

Search LEAP Wiki

5 Things you may not know about the PAL

Five things about the PAL

If you don't purchase your PAL from LEAP, then it's just another autosampler

Glossary

Glossary

Videos

LEAP's PAL Application Videos on YouTube

As Seen at LABAutomation 2011 in Palm, Springs, CA

At LabAutomation 2011 LEAP premiered a fast, super accurate Micro Dispenser for 96-, 384-, 1536-, 3456- plate work; up to 5 channels with individual feeds for enormous flexibility

• PAL sampling device customization and multiplexing software.
  
• PAL Accessories offering diverse applications including semi-solid metering, gravimetric determinations with balances and solid phase extractions.
  
• LEAP Automated Dried Blood Spot Extraction(DBS) robot elutes blood directly into LC
  

As Seen at AAPS 2010 in New Orleans Booth

• LEAP displayed the all new LEAP PAL-xt versions as the only authorized American dealer of CTC Analytics
• Flex-a-PAL Flexar PKI pump – PAL combination for UHPLC-MS
• LEAP Automated Dried Blood Spot Extraction(DBS) robot elutes blood directly into LC
  

As seen at Eastern Analytical Symposium (EAS) 2010

• LEAP displayed the all new LEAP PAL-xt versions as the only authorized American dealer of CTC Analytics
• Super fast Micro GC combined with COMBI PAL-xt; replaces 2-3 GCs to do the same sample load, saves power, time, down time – Falcon CALIDUS
• Flex-a-PAL Flexar PKI pump – PAL combination for UHPLC-MS

As Seen at CPSA October 2010

LEAP Technologies presented "Novel Walk Away Automation for DBS sample extraction from 4-Spot Cards to LC-MS" at the Chemical & Pharmaceutical Structure Analysis (CPSA) 2010 annual meeting held in Pennsylvania. The Dry Blood Spot (DBS) technique of eluding the blood sample from filter paper cards still lacks simple, reliable and inexpensive automation until today with LEAP Technologies and the PAL Platform. There are approximately 100 methods/applications known for these sample carrier DBS cards with many more being developed everyday. In many such areas automation will be a tremendous benefit. It will not only be labor saving, but also decrease handling, which means less errors and more consistency in getting the samples introduced into a separation and then the analytical system. The work flow of the automation approach step by step was discussed. The experience with hardware and software so far was be reviewed, including experience with the built-in internal standard addition. The SCAP DBS has patent pending technology applied and is simple to use. The approach of eluting the dry blood sample off the card directly to a multi-column system by-passes all manual and cumbersome treatment steps of the sample cards. First results from needed cycle times and throughput were presented. This is LEAP's latest approach to automated DBS using the PAL platform.

Novel Walk Away Automation for DBS Sample Extraction from 4-Spot Cards to LC-MS Authors: Brian Peat, Peter Smith, Dave Holub, LEAP Technologies, Inc, PO Box 969, Carrboro, NC 27510 Chemical & Pharmaceutical Structure Analysis (CPSA)

To see additional information please use our quick link: LEAP Automated Dried Blood Spot Extraction

As Seen at the ASMS June 2010

LEAP Technologies presented "A fully automated system for Hydrogen Deuterium Exchange" at the 58th ASMS Conference on Mass Spectrometry and Allied Topics (ASMS) in Salt Lake City, Utah.
To see information about this system please follow our quick link HD/X Applications

LEAP Technologies also presented "DLW (Dynamic Load and Wash) for Fast and Clean LC/MS Loading" The DLW,Dynamic Load and Wash, is designed for today’s most sensitive MS instruments this completely new injection and wash concept gives Near Zero Carryover and can drastically reduced cycle times. The linked combination of the two steps which are usually separate - minimizes cycle time and carry over. The DLW,Dynamic Load and Wash, is designed to be used on the PAL line of robots at the 58th ASMS Conference on Mass Spectrometry and Allied Topics (ASMS) in Salt Lake City, Utah.
To see information about this system please follow our quick link DLW

LEAP Technologies also presented the "Collect PAL"Fraction Collector which features “0” sample loss during collection,“0” well-to-well carry over,“0” drips and spills between fraction sites, “0” loss due to evaporation at the 58th ASMS Conference on Mass Spectrometry and Allied Topics (ASMS) Salt Lake City, Utah.
To see information about this system please follow our quick link Collect PAL

LEAP Technologies also presented the "Sample preparation automation to produce consistent and superior reliably coated tissue sections for MALDI Imaging MS " with the TM-Sprayer that delivers a coating of Matrix and Reagents at the 58th ASMS Conference on Mass Spectrometry and Allied Topics (ASMS) Salt Lake City, Utah.
To see information about this system please follow our quick link TM-Sprayer

As Seen at the Mid Atlantic LRIG May 2010

LEAP Technologies presented "A fully automated system for reconstituting semi-solid samples" at the Laboratory Robotics Interest Group Mid Atlantic Sixteenth Annual Technology Exhibition & Presentations (LRIG) in New Brunswick, NJ.
To see information about this system please follow our quick link Semi-Solid Applications

Consumables and Small Parts

Proud to reliably provide CTC/LEAP autosampler parts and consumables. In 2010 LEAP Technologies spun the small parts and consumable products business into our Company. Our mission is to enhance and ease the day to day operations of many of the world's scientific community. We recognize their work is some of the most challenging and productive in the world. We are privileged to count many as our customers.

LEAP's PAL Parts

 ↳ leaptec.com
 ↳ leapwiki.com

LEAP Technologies provides fully automated sample processing workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP also offers process control systems and stand alone workstations. LEAP is an open, neutral resource for technical support of existing PAL. Keep your PAL autosampler in peak working order LEAP offers full support and service for the PAL platform with over 11 years of history with the PAL. LEAP can also write custom macros, cycles, and scheduling software for your specific applications. We're proud that LEAP is known as a productivity improver for laboratories including government labs in agencies such as FDA, USDA, EPA, FBI, Armed Forces, Homeland Security and others function more efficiently. Robotic sample prep equipment works well in government labs and challenges lab technicians to keep feeding those more samples. Analytical equipment such as chromatographs and mass spectrometers are expensive devices and also expensive to maintain. LEAP can help to keep them running and analyze samples around the clock. LEAP has most of its laboratory automation robots under GSAcontract and is eager to service and maintain everything it sells to keep your expensive lab equipment running around the clock. CTC Analytics Combi PAL and HTS PAL for Front-End Automation for LC and GC applications. LEAP offers customized on-site training. LEAP Is continuing to develop FAQ, Solutions, Tips & Techniques,a Learning Center with the LEAP Wiki for the benefit of all PAL Platform Robotic users. We will continue exceeding customer expectations for quality, cost effective and efficient results using the most current technology and expertise in our industry. Our aim is to be the ultimate knowledge base on everything related to the PAL. LEAP is known as the autosamplerguys being in business over twenty years. LEAP has extensive multi-vendor experience with integrating the PAL. HTX, HTS, HTC, HTX-xt, HTS-xt, HTC-xt, LC-Mini, Combi PAL,COMBI-xt PAL, and GC PAL models. We have the Quality, Training, Parts availability, Infrastructure, and Experience.LEAP is sometimes referred to as an Application Automation House who is continually adding new products and solutions to its Lab Automation line. As we do so we change the content of this web site so please check back.
Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions whether it is our standard applications or customized to your needs. The Combi-xt PAL autosampler is able to perform liquid and headspace analysis. Headspace options include Solid Phase Micro-extraction (SPME) and ITEX-2 as alternative to purge and trap. The HTS-xt PAL autosampler dramatically increases sample throughput by offering automated sample prep, Peltier cooled and heated sample storage using the cooled stack, Peltier cooled and heated sample storage using the cooled stack and low carryover due to the self-cleaning DLW (Dynamic Load and Wash) module and L-Mark syringes, peltier cooled valves and columns. LEAP provides automation that fits your needs and reduces errors, increases sample capacity, increases efficiency, reduces cost by supplying long term productivity improvements with user friendly software and flexible hardware. LEAP performs just in time or on time sample prep and inject. LEAP offers full support of integrated and non-integrated PAL software with both sales and support for Cruise Control, Cycle Composer and Cycle Composer Macro Editor, Agilent ChemStation CTC Control, Mass Hunter and EZChrom CTC integration, Xcalibur, Waters Masslynx or Analyst CTC Control software, Thermo (Finnegan) XCalibur, LEAP Shell, and Chronos software. LEAP has many new offerings including PAL XCHANGE Option for PAL-xt Systems with park station,plunger coupling,active needle guide, XCHANGE syringes at www.palxchange.com The PAL is more than just an autosampler, it is a high-quality and efficient laboratory robot platform that can be customized by LEAP Technologies to meet your specific needs. The LEAP Wiki is a knowledge base of some applications for the LEAP CTC PAL.

Contact LEAP

Contact LEAP For additional information about LEAP and the PAL Platform, please contact LEAP Technologies.

The information and references contained in this web site are believed to be accurate and the most current information available.