On-line SPE LC/MS/MS to Detect Organonitrogen and Triazine Pesticides in Drinking Water


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On-line Solid Phase Extraction (SPE) coupled to Liquid Chromatography with tandem Mass Spectrometry (LC/MS/MS) to detect organonitrogen and triazine pesticides at concentrations of 10ng/L in drinking water.

Automated SPE sample clean-up and pre-concentration step utilizing large volume injection (5mL of untreated water samples) prior to LC/MS/MS analysis. With this approach, all pesticides are first trapped on an SPE cartridge and then back flushed onto a reverse phase LC column. The pesticides are then separated on the analytical column using a binary LC gradient and are determined on the ABI LC/MS/MS System.

5mL of tap water were loaded onto a on-line SPE cartridge using a CTC HTC PAL autosampler (fitted with a 5mL syringe, a 5mL loop and a 32 position 10mL sample rack). The trap cartridge was fitted into load position A of a high pressure, PAL 10 port switching valve. An isocratic pump was used to load the sample at 3mL/min onto the trapping cartridge. After 2.5 minutes the valve was switched to position B where an Binary pump was used to provide the gradient elution. After 14.5 minutes the switching valve was switched back to the loading position to analyze the next sample.


Application Note

Courtesy ABI Application note for On-Line SPE LC/MS/MS of Pesticides
Application Note from www.appliedbiosystems.com

Project Ref: M-006
Inline Sample Concentration(M-006)


For other cost/time saving measures consider:

  • LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [1]
  • Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [2]
  • Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [3]
  • “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [4]
  • Dual Channel Parallel Injection (M-005) using LEAP Shell software [5]
  • Summary of LC Multi Valve Applications [6]
  • LEAP Shell Software and application for custom injections and scheduling [7]

Videos of PAL

LEAP's PAL Application Videos on YouTube

 ↳ leaptec.com  ↳ leapwiki.com

LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics’ PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.

Contact LEAP

Contact LEAP

For additional information about LEAP and the PAL Platform, please contact LEAP Technologies.

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