Kinetic Reaction with GC Injection


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The purpose of this application is the investigation of the reaction kinetics of incubated samples. Samples in 2mL vials are incubated at higher temperatures that start a chemical reaction. After expiration of a Sampling Time, a 4uL aliquot of sample is aspirated from each vial in the incubator and quenched by 10mL of methanol. The number of samplings per 2mL incubation vial can range from 6-9. Four different macros are provided one for each number of samplings. The length of the overall reaction time determines the length of Sampling Time intervals and what can be accomplished during those intervals. For long reaction times (Long-RT), samples are not only be drawn and quenched but also injected into a GC. Shorter Sampling Time intervals are typical for short reaction times (Short-RT), which leaves only time for sample drawing and quenching. In the latter case, sample injections need to be performed after all samples are drawn and quenched using a regular liquid GC injection macro. For both long and short reaction times, samples are being put into the incubator in a staggered fashion. The staggering interval needs to be long enough that either all autosampler actions related to drawing and quenching of one sample can be completed (Short-RT) or a GC run including cool down can be performed (Long-RT). Lastly, the number of 2mL vials that can be processed in a staggered fashion depends on how the 2mL incubation vial staggering interval compares to the sampling time interval, e.g., how many staggering intervals “fit” into one Sampling Time interval.

Project Ref: C1027

For other cost/time saving measures consider:

  • LEAP Shell Software and application for custom injections and scheduling [1]

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LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics’ PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.

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For additional information about LEAP and the PAL Platform, please contact LEAP Technologies.

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