Twin PAL - High Throughput injections using Cycle Composer
The HTX Twin PAL is used to increase throughput. It approximately doubles the injection rate of samples. Each PAL is controlled from its own session of Cycle Composer Software assigned to a different COM port. The application uses an LC injection macro onto 1 valve, using shared samples. PAL1 injects first and alternates samples with the PAL2. Both PALs check for a ready signal from the mass spec (INJECT), and use special Sync signals to check the status of the other PAL before accessing the shared areas.
In order to maximize the speed of injections, valve washes are handled in an unusual way. Both PALS are injecting samples onto a single valve, the syringe of one PAL is filled with wash solvent while the other PAL is injecting, and washes the injection port while the first PAL is washing its syringe.
Data System is Xcalibur
Project Ref: T1013
The HTX Twin PAL is used to increase throughput. It approximately doubles the injection rate of samples by staggering injections onto 2 sets of column switching setups totaling in 4 LC columns and 5 valves. Four customer-provided pumps will be used in a dedicated fashion, i.e., there are 2 elution and 2 regeneration pumps. Each PAL is controlled from its own session of Cycle Composer assigned to a different COM port.
Data is collected for several samples in one data file in order to further increase throughput. Both PALs have access to all available stacks and samples which can be “cherry-picked” if desired. Only one PAL checks for a ready signal from the mass spec. Special Sync signals are used to check the status of the other PAL before accessing shared areas.
In order to maximize the speed of injections, valve washes are handled by two Valve Self-Washing Systems.
Project Ref: T1002
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve 
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE 
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst 
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software 
- Dual Channel Parallel Injection (M-005) using LEAP Shell software 
- Summary of LC Multi Valve Applications 
- LEAP Shell Software and application for custom injections and scheduling 
Videos of PAL
LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics’ PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
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For additional information about LEAP and the PAL Platform, please contact LEAP Technologies.