Single Drop Micro Extraction

From LEAP

Revision as of 15:52, 25 October 2012

	GC Headspace
	Application Type
	STANDARD
	Application ID
	GCSDME
	Description
	GC Single Drop Micro Extraction sample processing with ChemStation.

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Single Drop Micro Extraction (SDME)

Single drop micro extraction (SDME) has emerged as one of the simplest and most easily implemented forms of micro-scale sample cleanup and pre-concentration. An ordinary PAL Gas Chromatography syringe is used to suspend micro liter quantities of extracting solvent either directly immersed in the sample, or in the headspace above the sample. The syringe is then used to inject the solvent and extracted analytes into the Gas Chromatography system for identification and/or quantitation.

SDME is a straightforward technique in which a micro drop of solvent is suspended from the tip of a conventional GC syringe and then is immersed in a sample solution in which it is immiscible or suspended in the headspace above the sample.

Headspace SDME is similar to traditional headspace sampling in that volatiles are sampled from the vapors above the sample, thus avoiding interferences from the sample matrix.

Headspace SDME is similar to SPME but the fiber used in SPME is replaced by a liquid micro drop.

Advantages of the technique include high selectivity, good quantitation, low detection limits, no carryover, higher upper limits of detection and minimal sample preparation. One of the biggest advantages is you only need one drop of a solvent instead of a SPME fiber. HS-SDME is found to provide excellent clean up for samples with complicated matrices.

Like with SPME, you can perform the SDME technique manually. And once you’ve worked out your method,you can automate it with your Combi PAL autosampler.

SDME, using typically 1–3 lL of an organic solvent at the tip of a microsyringe. After extraction, the microdrop is retracted back into the syringe and transferred for further analysis.

There are currently two main approaches can be used to perform SDME with the PAL: (1) Direct Immersion (DI)-SDME (2) Headspace (HS)-SDME

In DI-SDME, a drop of a water-immiscible solvent is suspended directly from the tip of a microsyringe needle immersed in the aqueous sample.

Photos

PAL Single Magnet Mixer	PAL Single Position Stirrer installed on PAL	Sample vial tray	Magnetic tool tip
Bar code reader	Agitator	Agitator	Vial in transfer
Agilent 6890 GC / 5975C VL MSD

Accessories

Single Magnet Mixer
Agitation_of_Samples
Multi_Headspace_Extractions
ITEX-2
Vent Tool
Bar Code Reading
CombiPAL

Software

Chronos Mastersoftware for organizing parallel processes for sample preparation

Useful links

General Information on Gas Chromatography

Videos

Movie of the COMBI PAL with Headspace in action.

Videos of PAL

LEAP's PAL Application Videos on YouTube

Info on Chronos software from LEAP Technologies
Chronos Mastersoftware is an ideal solution for automating complex GC Sample Preparation steps on the CTC PAL. Software Integration with data systems ChemStation™ or Xcalibur™ assures seamless functionality with existing systems. Chronos not only exchanges information with other systems, but also controls the complete process and optimizes intelligent time-management resulting in significant improvement in system throughput.Single Sample List Control – including the flexible Twin PAL


Chronos Mastersoftware for organizing parallel processes for sample preparation

Keywords: Single drop micro extraction, Solvent micro extraction, Liquid-phase micro extraction, Gas Chromatography (GC), In headspace SDME or direct immersion two-phase SDME with a headspace above the sample, Headspace Liquid Microextraction, single-drop microextraction (SDME)

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LEAP Technologies provides fully automated sample processing workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP also offers process control systems and stand alone workstations. LEAP is an open, neutral resource for technical support of existing PAL. Keep your PAL in peak working order LEAP offers full support and service for the PAL platform with over 11 years of history with the PAL. LEAP can also write custom macros, cycles, and scheduling software for your specific applications. We're proud that LEAP is known as a productivity improver for laboratories including government labs in agencies such as FDA, USDA, EPA, FBI, Armed Forces, Homeland Security and others function more efficiently. Robotic sample prep equipment works well in government labs and challenges lab technicians to keep feeding those more samples. Analytical equipment such as chromatographs and mass spectrometers are expensive devices and also expensive to maintain. LEAP can help to keep them running and analyze samples around the clock. LEAP has most of its laboratory automation robots under GSAcontract and is eager to service and maintain everything it sells to keep your expensive lab equipment running around the clock. CTC Analytics Combi PAL and HTS PAL for Front-End Automation for LC and GC applications. LEAP offers customized on-site training. LEAP Is continuing to develop FAQ, Solutions, Tips & Techniques,a Learning Center with the LEAP Wiki for the benefit of all PAL Platform Robotic users. We will continue exceeding customer expectations for quality, cost effective and efficient results using the most current technology and expertise in our industry. Our aim is to be the ultimate knowledge base on everything related to the PAL. LEAP is known as the autosamplerguys being in business over twenty years. LEAP has extensive multi-vendor experience with integrating the PAL. HTX, HTS, HTC, HTX-xt, HTS-xt, HTC-xt, LC-Mini, Combi PAL,COMBI-xt PAL, and GC PAL models. We have the Quality, Training, Parts availability, Infrastructure, and Experience.LEAP is sometimes referred to as an Application Automation House who is continually adding new products and solutions to its Lab Automation line. As we do so we change the content of this web site so please check back.
Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions whether it is our standard applications or customized to your needs. The Combi-xt PAL autosampler is able to perform liquid and headspace analysis. Headspace options include Solid Phase Micro-extraction (SPME) and ITEX-2 as alternative to purge and trap. The HTS-xt PAL autosampler dramatically increases sample throughput by offering automated sample prep, Peltier cooled and heated sample storage using the cooled stack, Peltier cooled and heated sample storage using the cooled stack and low carryover due to the self-cleaning DLW (Dynamic Load and Wash) module and L-Mark syringes, peltier cooled valves and columns. LEAP provides automation that fits your needs and reduces errors, increases sample capacity, increases efficiency, reduces cost by supplying long term productivity improvements with user friendly software and flexible hardware. LEAP performs just in time or on time sample prep and inject. LEAP offers full support of integrated and non-integrated PAL software with both sales and support for Cruise Control, Cycle Composer and Cycle Composer Macro Editor, Agilent ChemStation CTC Control, Mass Hunter and EZChrom CTC integration, Xcalibur, Waters Masslynx or Analyst CTC Control software, Thermo (Finnegan) XCalibur, LEAP Shell, and Chronos software. The PAL is more than just an autosampler, it is a high-quality and efficient laboratory robot platform that can be customized by LEAP Technologies to meet your specific needs.

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