Kinetic Reaction with GC Injection

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Revision as of 22:42, 5 May 2009

Overview

The purpose of this application is the investigation of the reaction kinetics of incubated samples. Samples in 2mL vials are incubated at higher temperatures that start a chemical reaction. After expiration of a Sampling Time, a 4uL aliquot of sample is aspirated from each vial in the incubator and quenched by 10mL of methanol. The number of samplings per 2mL incubation vial can range from 6-9. Four different macros are provided one for each number of samplings. The length of the overall reaction time determines the length of Sampling Time intervals and what can be accomplished during those intervals. For long reaction times (Long-RT), samples are not only be drawn and quenched but also injected into a GC. Shorter Sampling Time intervals are typical for short reaction times (Short-RT), which leaves only time for sample drawing and quenching. In the latter case, sample injections need to be performed after all samples are drawn and quenched using a regular liquid GC injection macro. For both long and short reaction times, samples are being put into the incubator in a staggered fashion. The staggering interval needs to be long enough that either all autosampler actions related to drawing and quenching of one sample can be completed (Short-RT) or a GC run including cool down can be performed (Long-RT). Lastly, the number of 2mL vials that can be processed in a staggered fashion depends on how the 2mL incubation vial staggering interval compares to the sampling time interval, e.g., how many staggering intervals “fit” into one Sampling Time interval.

Project Ref: C1027

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For additional information about this technique please contact LEAP Technologies for detailed information

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