Pooling of vial contents for QC testing
From LEAP
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| - | The long rail of the HTX | + | [[Image:Pooling.png |right|200px|thumb|Pooling of samples]] Pooling of individual samples can provide a robust and efficient way to perform some experiments. Pooling of samples can be an effective means to increase cost-effectiveness in routine surveillance and exposure assessment assays.<br><br> |
| + | The long rail of the [http://leapwiki.com/mediawiki/index.php?title=Category:HTX HTX] [http://leapwiki.com/mediawiki/index.php?title=Category:PAL PAL] is used to mount 8 standard [http://www.leapwiki.com/mediawiki/index.php?title=Trayholder ambient tray holders]. The system uses a [http://www.leapwiki.com/mediawiki/index.php?title=Large_Volume_Dispensing#Side_Port_Syringe_Accessory side-port syringe] and is designed to pierce the vial septa, go to the bottom of the 5 mL vial and wait while the in-house vacuum evacuates the contents to a flask which pools contents from all of sample vials for later testing. This application was used to batch-test vials filled with injection grade water. The task was previously performed manually. | ||
| - | The time that the instrument can stay in the aspirate position can be adjusted by the user to ensure complete recovery of the vial contents. In this case each vial contains 0.85ml of sample and a minimum of 0.7ml needs to be extracted. Total capacity of 400 vials. Custom trays have been drilled so that the vials are held at a 15 degree angle, allowing for maximum recovery from each vial. If more than 400 vials are needed for pooling, the customer will re-fill the tray holders with new racks at a prescribed time interval. The system can be reconfigured to handle other vials sizes. | + | The time that the instrument can stay in the aspirate position can be adjusted by the user to ensure complete recovery of the vial contents. In this case each 5 mL vial contains 0.85ml of sample and a minimum of 0.7ml needs to be extracted. Total capacity of 400 vials. [http://leapwiki.com/mediawiki/index.php?title=Unique_Storage Custom trays]have been drilled so that the vials are held at a 15 degree angle, allowing for maximum recovery from each vial. If more than 400 vials are needed for pooling, the customer will re-fill the tray holders with new racks at a prescribed time interval. The system can be reconfigured to handle other vials sizes. |
| - | [[Image:Pooling_of_vials_PAL.png|center]] | + | [[Image:Pooling_of_vials_PAL.png|600px|thumb|center|LEAP CTC Robotic Pooling ]] |
'''Project Ref: H1115''' | '''Project Ref: H1115''' | ||
| - | === For other cost/time saving measures | + | [[Image:15 deg A.png|center|600px|thumb|center|Close up of Pooling Trays]] |
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| + | '''Potential Benefits:''' | ||
| + | * Cost Reduction | ||
| + | * Collective Testing | ||
| + | |||
| + | By pooling samples you are obtaining information from a greater number of samples for much less cost.<br> | ||
| + | |||
| + | ''' Photos ''' | ||
| + | <gallery> | ||
| + | Image:Pooling_of_vials_PAL.png | ||
| + | Image:15 deg A.png| Close up of tray | ||
| + | Image:Pooling.png | pooling of samples | ||
| + | </gallery> | ||
| + | |||
| + | ''' Other Pooling Information on Web '''<br> | ||
| + | [http://www.ncbi.nlm.nih.gov/pubmed/15914576?log$=activity Web Example of Pooling of Samples] | ||
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| + | [http://www3.interscience.wiley.com/journal/112636968/abstract?CRETRY=1&SRETRY=0 Pooling of Samples on Web] | ||
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| + | ''' For other cost/time saving measures consider: '''<br> | ||
[[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Main_Page#Examples_of_Special_Solutions Other Examples of Customers Solutions]<br> | [[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Main_Page#Examples_of_Special_Solutions Other Examples of Customers Solutions]<br> | ||
[[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=LC_Multi-Valve_Applications Summary of LC Multi Valve Applications]<br> | [[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=LC_Multi-Valve_Applications Summary of LC Multi Valve Applications]<br> | ||
[[image: System Icon.png|40px]] [http://www.leaptec.com/software/leap-shell-3.php LEAP Shell Software and application for custom injections and scheduling]<br> | [[image: System Icon.png|40px]] [http://www.leaptec.com/software/leap-shell-3.php LEAP Shell Software and application for custom injections and scheduling]<br> | ||
[[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Glossary Glossary]<br> | [[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Glossary Glossary]<br> | ||
| - | [[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Main_Page | + | [[image: System Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Main_Page LEAP Wiki Main Page]<br> |
---- | ---- | ||
| + | === Videos of PAL === | ||
| + | <br> | ||
| + | [[Image:Movie Icon.png|40px]] | ||
| + | [http://www.youtube.com/user/LEAPTechnologies#g/u LEAP's PAL Application Videos on YouTube] | ||
| + | <br> | ||
{{logo}} | {{logo}} | ||
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LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics’ PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis. <br> | LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics’ PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis. <br> | ||
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions. | LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions. | ||
Current revision
The long rail of the HTX PAL is used to mount 8 standard ambient tray holders. The system uses a side-port syringe and is designed to pierce the vial septa, go to the bottom of the 5 mL vial and wait while the in-house vacuum evacuates the contents to a flask which pools contents from all of sample vials for later testing. This application was used to batch-test vials filled with injection grade water. The task was previously performed manually.
The time that the instrument can stay in the aspirate position can be adjusted by the user to ensure complete recovery of the vial contents. In this case each 5 mL vial contains 0.85ml of sample and a minimum of 0.7ml needs to be extracted. Total capacity of 400 vials. Custom trayshave been drilled so that the vials are held at a 15 degree angle, allowing for maximum recovery from each vial. If more than 400 vials are needed for pooling, the customer will re-fill the tray holders with new racks at a prescribed time interval. The system can be reconfigured to handle other vials sizes.
Project Ref: H1115
Potential Benefits:
- Cost Reduction
- Collective Testing
By pooling samples you are obtaining information from a greater number of samples for much less cost.
Photos
|
Close up of tray |
pooling of samples |
Other Pooling Information on Web
Web Example of Pooling of Samples
For other cost/time saving measures consider:
Other Examples of Customers Solutions
Summary of LC Multi Valve Applications
LEAP Shell Software and application for custom injections and scheduling
Glossary
LEAP Wiki Main Page
Videos of PAL
LEAP's PAL Application Videos on YouTube
LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics’ PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
Contact LEAP
 Contact LEAP
For additional information about LEAP and the PAL Platform, please contact LEAP Technologies. |
