M-003 Dual Channel Parallel Injection - Cycle Composer
From LEAP
| LC Dual Stream Staggered Injections |
| Application Type | |
| SPECIAL | |
| Application ID | |
| M-003 | |
| Description | |
| High Throughput LC injections alternating onto two analytical columns. |
Contents |
Overview
This configuration allows shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating the alternating column during the sample acquisition. It uses
Cycle Composer Software - this application is run with Standard LEAP macros outside of Analyst® Software from Applied Biosystems Inc®
If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.
This application is useful to increase sample throughput by taking advantage of front and/or back-cut time to load the next sample in the sequence early. It requires that the "period of interest" in the chromatography is significantly shorter than the overall chromatographic run-time. There are two columns that are running in "staggered" mode, where samples are pre-loaded onto the other channel while the previous channel is still in acquisition. There is no pre-column. The gradient programs regenerate the analytical columns in each stream.
Please note: LEAP can not control the MS Selector valve as part of the cycle.
If injections are staggered in the following way, there is a potential for up to 100% gain:
Hardware Requirements
- DoubleStack multiple valve system and a 6 port injection valve with FWS in front of it is required
| Key Benefits |
| Saving of Front and Back cut |
| Performs "Look Ahead" |
| Staggering of Injections |
| Restrictions |
| Requires matching sample lists in Cycle Composer and Analyst |
| Columns may perform slightly different on alternating samples |
| Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
|---|---|---|---|---|---|---|
| M-003 | Dual Channel Parallel Injection Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition | Cycle Composer outside of Analyst | * DoubleStack valve * 6 port injection valve * FWS in front of 6 port valve * Gradient Pump * Regeneration pump | * Saving of Front and Back Cut * Provides "Look Ahead" * Provides staggering of injections | * Requires matching sample lists in Cycle Composer and Analyst * Columns may perform slightly differently on alternating samples | No |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [1]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [2]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [3]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [4]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [5]
- Summary of LC Multi Valve Applications [6]
- LEAP Shell Software and application for custom injections and scheduling [7]
This workstation application is based on the CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your LC or GC requirements. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible automation.
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