ITSP cartridge
From LEAP
| Sample Filtration |
| Application Type | |
| SPECIAL | |
| Application ID | |
| ITSP | |
| Description | |
| Sample Filtration with ITSP System of Microliter Inc. |
Contents |
Overview
ITSP (Instrument Top Sample Prep) for Serial Solid Phase Extraction (SPE) or Sample Filtration uses a standard PAL syringe as the solvent reservoir for Microliter's disposable SPE cartridges. The combination replaces expensive dedicated SPE liquid handlers and cumbersome or inconsistent vacuum manifolds. Pressurizing multiple wells is eliminated, no loss of samples or inconsistency of extraction due to leaks or loss of vacuum. Furthermore standard SPE liquid handlers require a manual transfer of the extracted samples to a sample injector. Since the PAL itself is a sample injector this can be avoided using the automated ITSP – PAL combination.
Significant Markets
Any labs who are doing High Performance Liquid Chromatography (HPLC) or more significantly, Ultra High Performance Liquid Chromatography (UHPLC) analysis. It is imperative that particles are eliminated to avoid blockage of HPLC lines and columns.
How it works by Microliter
ITSP (Instrument Top Sample Prep) is a consumable device that allows for a common autosampler used on most chromatographs to prepare client samples using Solid Phase Extraction (SPE) or filtration. ITSP’s septum seals and grips the needle creating a closed environment above the bedmass. By removing the volume above the column bedmass and replacing it with the analytical instrument’s syringe, solutions can be passed across the bedmass using the hydraulic pressure of the syringe plunger. The gripping action of the septum also allows for the ITSP device to be transported to any location on the autosampler. By defining waste and elution locations the device can be prepped in one location and moved to elute the collected compounds in another. Once eluted into a clean receptacle the instrument can discard the device and inject the eluate onto the analytical instrument. ITSP cartridges are available in a wide range of stationary phases, including reverse phase, normal phase and ion exchange phases. The ITSP filtration cartridges (0.45µm) are available with different filtration membranes, including, PTFE, Nylon and PVDF.
The following diagrams illustrate the ITSP device and the typical set-up on an LC/MS/MS autosampler.
ITSP(Instrument Top Sample Prep) for Serial Solid Phase Extraction or Sample Filtration uses a standard PAL syringe as the solvent reservoir for Microliter's disposable SPE cartridges. The combination replaces expensive dedicated SPE liquid handlers and cumbersome or inconsistent vacuum manifolds. Pressurizing multiple wells is eliminated, no loss of samples or inconsistency of extraction due to leaks or loss of vacuum. Furthermore standard SPE liquid handlers require a manual transfer of the extracted samples to a sample injector. Since the PAL itself is a sample injector this can be avoided using the automated ITSP – PAL combination. ITSP for PAL Systems is distributed exclusively by Microliter Inc.
Brochure
ITSP Sample Prep Automation Guide
Video
Movies of ITSP on PAL in action.
Movie of ITSP on PAL in action.
Other Reference Material
2010 Method Development Poster ALA
Example Application Notes
Determination of Cocaine and Benzoylecgonine in Human Urine Using Automated ITSP Solid Phase Extraction and Liquid Chromatography Mass Spectrometric Detection
ITSP Extraction of Cortisol from Plasma
Autosampler-Based Sample Preparation for Just-in-Time Extraction of Tacrolimus, Sirolimus and Cyclosporine for LC/MS Analysis
AMHSA-5_Drugs_of_Abuse_ITSP_Application_Notes
Automated SPE-LC/MS/MS Assay for 25-OH Vitamin D Metabolites from Serum
Determination of Vitamin D Metabolites in Human Serum Using Automated ITSP Solid Phase Extraction and Liquid Chromatography-Tandem Mass Spectrometry
Development of a SPE/DART Assay for Rapid Narcotics Screening in Urine
An Automated Procedure for Determining ITSP SPE Analytical Parameters for the Analysis of Cortisol and Cortisone in Urine
Integrated SPE/LC/MS/MS Analysis of Small Molecules in Biological Samples
ITSP Hardware Kits and Accessories
ITSP Hardware Kits offer you the proper hardware components to manage ITSP. Included in each kit is the Prep Tray, Waste Receptacle, a Needle Guide and a replacement screw for the LC inlet.
If you are using ITSP for Solid Phase Extraction you may also need additional software, solvent reservoirs, a fast wash station or other sample trays. In this section you will see a variety of solutions for these additional needs.
Accessories for the PAL
Other Accessories for PAL Robots
Videos of PAL
LEAP's PAL Application Videos on YouTube
LEAP Technologies provides fully automated sample processing workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP also offers process control systems and stand alone workstations. LEAP is an open, neutral resource for technical support of existing PAL. Keep your PAL in peak working order LEAP offers full support and service for the PAL platform with over 11 years of history with the PAL. LEAP can also write custom macros, cycles, and scheduling software for your specific applications. We're proud that LEAP is known as a productivity improver for laboratories including government labs in agencies such as FDA, USDA, EPA, FBI, Armed Forces, Homeland Security and others function more efficiently. Robotic sample prep equipment works well in government labs and challenges lab technicians to keep feeding those more samples. Analytical equipment such as chromatographs and mass spectrometers are expensive devices and also expensive to maintain. LEAP can help to keep them running and analyze samples around the clock. LEAP has most of its laboratory automation robots under GSAcontract and is eager to service and maintain everything it sells to keep your expensive lab equipment running around the clock. CTC Analytics Combi PAL and HTS PAL for Front-End Automation for LC and GC applications. LEAP offers customized on-site training. LEAP Is continuing to develop FAQ, Solutions, Tips & Techniques,a Learning Center with the LEAP Wiki for the benefit of all PAL Platform Robotic users. We will continue exceeding customer expectations for quality, cost effective and efficient results using the most current technology and expertise in our industry. Our aim is to be the ultimate knowledge base on everything related to the PAL. LEAP is known as the autosamplerguys being in business over twenty years. LEAP has extensive multi-vendor experience with integrating the PAL. HTX, HTS, HTC, HTX-xt, HTS-xt, HTC-xt, LC-Mini, Combi PAL,COMBI-xt PAL, and GC PAL models. We have the Quality, Training, Parts availability, Infrastructure, and Experience.LEAP is sometimes referred to as an Application Automation House who is continually adding new products and solutions to its Lab Automation line. As we do so we change the content of this web site so please check back.
Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions whether it is our standard applications or customized to your needs. The Combi-xt PAL autosampler is able to perform liquid and headspace analysis. Headspace options include Solid Phase Micro-extraction (SPME) and ITEX-2 as alternative to purge and trap. The HTS-xt PAL autosampler dramatically increases sample throughput by offering automated sample prep, Peltier cooled and heated sample storage using the cooled stack, Peltier cooled and heated sample storage using the cooled stack and low carryover due to the self-cleaning DLW (Dynamic Load and Wash) module and L-Mark syringes, peltier cooled valves and columns. LEAP provides automation that fits your needs and reduces errors, increases sample capacity, increases efficiency, reduces cost by supplying long term productivity improvements with user friendly software and flexible hardware. LEAP performs just in time or on time sample prep and inject. LEAP offers full support of integrated and non-integrated PAL software with both sales and support for Cruise Control, Cycle Composer and Cycle Composer Macro Editor, Agilent ChemStation CTC Control, Mass Hunter and EZChrom CTC integration, Xcalibur, Waters Masslynx or Analyst CTC Control software, Thermo (Finnegan) XCalibur, LEAP Shell, and Chronos scheduling software. The PAL is more than just an autosampler, it is a high-quality and efficient laboratory robot platform that can be customized by LEAP Technologies to meet your specific needs.
Contact LEAP
 Contact LEAP
For additional information about LEAP and the PAL Platform, please contact LEAP Technologies. |
The information and references contained in this web site are believed to be accurate and the most current information available.
