Prepare and Analyze a Plant Extract by GC

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{{LSApp
{{LSApp
   |name        = Plant Extract
   |name        = Plant Extract
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   |image      = PolymerTwinGC.png
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   |image      = PlantExtract1.png
   |type        = Sample Prep and Inject
   |type        = Sample Prep and Inject
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   |id          = Polymers1001
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   |id          = Plant1001
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   |description = Polymer Analysis
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   |description = Analyzing a Plant Extract for Chemicals
}}
}}
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{{logo}}
=== Overview ===
=== Overview ===
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Chemical analysis of polymers, as well as other forms of analysis such as viscosity and other tests are a necessary part of polymer development, as well as QC processes during manufacture. Polymers are generally solids when in the final product, and thus are not amenable to such analysis unless they can be first liquefied. Preparation for analysis requires multiple stages of treatment frequently with heated reaction and cooling steps prior to introduction into a detector such as a GC. Such processes are lengthy, time-critical and labor intensive to perform manually. They frequently involve handling of caustic or toxic reagents. There is a strong case therefore, for automation of such processes.
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Plant material and biomass in general are not just for food and feed any longer. They have become the feedstock for fuel, neutraceuticals, drugs and food supplements such as OMEGA-3 or other strong antioxidants. There is a growing research community that looks from a genetics and maximum yield perspective at many bio-organism. A primary example are seeds for oil or algae for OMEGA-3. All these examples have one complication in common: Before analysis for the presence of certain chemicals (molecules) an extract has to be generated out of the raw sample. Fibers, skin and other natural tissue has to be filtered and many constituants of the plants that are of interest have to be extracted in an extraction and separation process. There are many procedures that use a manual approach. As long as the methodology of plant research is its trial and error stage automation can't be justified. However, once a systematic work flow is well defined an automated process is not only more productive but leads to more consistency and less wasteful use of materials, disposables, solvents and of course highly educated personnel's time.
 +
[http://www.leaptec.com/ LEAP Technologies] had many customers that successfully got their extractions automated. Some started out with Seeds, Algae, Tobacco and other leafy plants. The automation was in many cases "just-in time" sample preparation on [http://www.leaptec.com/ LEAP's] primary XYZ platform the CTC PAL with a subsequent injection onto a [http://leapwiki.com/mediawiki/index.php?title=Category:Gas_Chromatography Gas Chromatograph] or [http://www.leapwiki.com/mediawiki/index.php?title=Category:Mass_spectrometry Mass Spectrometer].
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LEAP has broken new ground in one such application, where the complexities of this type of sample preparation have been fully automated using an HTX2H in the configuration shown below:
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Often a [http://www.leapwiki.com/mediawiki/index.php?title=TwinPAL GC Twin PAL] accomplishes the extraction and filtration before a GC analyzes plant extract material. The application is to take a vial containing plant material, add [http://www.leapwiki.com/mediawiki/index.php?title=Large_Volume_Dispensing solvent], [http://www.leapwiki.com/mediawiki/index.php?title=Agitation_of_Samples heat & agitator], [http://www.leapwiki.com/mediawiki/index.php?title=Filtering_of_Samples filtering], and then analyze on a 7890 GC.  This application may be applicable to any customer who wants to do filtration providing the liquid can be pushed thru the filter plate with a low pressure inert gas through the headspace syringe.
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=== Significant Markets  ===
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Additional accessories such as an integrated [http://www.leapwiki.com/mediawiki/index.php?title=Balance_PAL balance] and a [http://leapwiki.com/mediawiki/index.php?title=Bar_Code_Reading barcode scanner] have been added in the past.
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* Polymer Manufactures
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* Biodiesel Blenders
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'''Markets Served:'''<br>
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Elements of this application can be applied to labs requiring any type of filtration of a sample while maximizing throughput.
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* University
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* Research
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* FDA
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* EPA
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* USDA
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* Polymer Distributors
 
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* Polymer End Users
 
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'''What is a Polymer experiment?'''<br/>
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'''What is a Plant Extract experiment?'''<br/>
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The sample preparation process involves 2 heated reactions with cooldown steps in between. The first hydrolyses the solid polymer in a mixture with an internal standard at high temperature in Agitator 1. The pellets dissolve and after a quenching reaction and cooldown period, a derivitizing reagent is added followed by another heated incubation at lower temperature. The reactants are then injected onto a GC for analysis.
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The sample prep procedure is quite simple with the exception of the final sample needing to not contain any of the plant material when it gets to the analysis procedure. The samples are incubated in a [http://www.leapwiki.com/mediawiki/index.php?title=Agitation_of_Samples heated agitator], and then allowed to settle. The sample solvent, possible containing small pieces of plant material, is filtered. LEAP has a variety of ways to filter whether it is thru a 96 well filter plate or with Uni-Prep Vials. See [http://www.leapwiki.com/mediawiki/index.php?title=Filtering_of_Samples filtering of samples] for the different approaches.  
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A balance is used to check that a minimum weight of product is in the original vial. If not, the sample is skipped. The samples are barcoded at the time the sample list is set up. At runtime the scanned barcode is compared to the expected barcode and if there is a mis-match then the sample is also skipped. .
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LEAP Shell handles the sample scheduling of each of the 5 stages of the process, overlapping them all and filling up both agitators according to the available time between operations. It also handles barcode and weight validation as well as data logging.  
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'''LEAPS Approach'''<br/>
'''LEAPS Approach'''<br/>
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• Twin PAL control using a single sample list in LEAP Shell.<br/>
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The GC [http://www.leapwiki.com/mediawiki/index.php?title=TwinPAL Twin PAL] in combination with the [http://www.leapwiki.com/mediawiki/index.php?title=Category:Chronos Chronos Mastersoftware] makes a very effective and flexible workstation. We were able to design and provide a solution which met the following challenges from the customer:<br/>  
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• Two heated zones for different temperature reactions.<br/>
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# Provide the necessary filtration to have clean samples to analyze. In order to get good blow-out of the liquid through the filter, it was determined that the filter plate should be sealed and have a small nozzle on the underside to ensure accurate collection without crossover to adjacent collection vials.<br/>
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• Direct injection to either of two GCs.<br/>
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# Throughput. By using a Twin PAL we are able to take advantage of having one PAL do the preparation while the other does the sample injections.<br/>
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• Balance integration for weighing of solid product and internal standard.<br/>
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# Spatial concerns. Provide a way to get everything that was required to fit. <br/>
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• Up to 8 reactant vessels, and 32 sample vial capacity.<br/>
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• Overlapped staggered processing of all samples for high throughput.<br/>
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• Barcode checking, mismatched vials are not processed.<br/>
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• Weight checking, over or under weight vials are skipped.<br/>
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• Data reporting to log files.<br/>
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• GC failsafe mode – if GC goes down during a run, samples are still processed.<br/>
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• Integrated with Chemstation.<br/>
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<br/>
<br/>
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'''Market potential:'''<br/>
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===  Photos ===
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The success of this initial installation shows LEAP’s capability to solve complex automation challenges in areas which have previously been out of our reach. The polymer industry presents opportunities to expand these types of sales, not only for these complex sample preparation applications for solids, but for simpler applications such as viscosity testing or sample prep for NMR.<br/>
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In more general terms, this application illustrates the power of LEAP Shell and the new capabilities it provides which can be applied much more widely. Consideration should be given to the following opportunities:<br/>
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• Handling of single or multiple overlapped incubations e.g. Headspace or SPME.<br/>
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• Extensive customization of the Sample list.<br/>
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• Open access – walk-up addition of samples during a run.<br/>
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• Extensive use of the “IF” statement to manage error conditions and determine criteria for sample processing or not.<br/>
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• Output of data to log files for record keeping or for integration with data systems.<br/>
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= Photos =
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<gallery>
<gallery>
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   Image:PolymerTwinGC.png| LEAP TWIN PAL for Polymers
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   Image:PlantExtract1.png| Automation of Plant Extract before Gas Chromatography
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  Image:Seed Crushing.png| Seed Crusher for hand use
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  Image:Seed_Filtration.png| LEAP Twin PAL with Agitator, 96 well plate filtration, large volume solvent,
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  Image:Filtration.png| Filtration with 96 well plate
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  Image:XPress_on_PAL.png | LEAP X-Press option on LEAP CTC PAL
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  Image:UniPrep_Filter_diagram.png | UniPrep Filter Diagram
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  Image:DISP_TOOL_Dilutor.png | Dilutor with DISPTool
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  Image:Dilutor.png | Dilutor on PAL
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  Image:Chronos_Sample_Sequence.png | Chronos software schedule
</gallery>
</gallery>
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<br>
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* Additional info on '''[http://www.leapwiki.com/mediawiki/index.php?title=Balance_PAL Integrated Balance]'''
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* Additional info on '''[http://leapwiki.com/mediawiki/index.php?title=Bar_Code_Reading Barcode Reading]'''
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* Additional info on '''[http://leapwiki.com/mediawiki/index.php?title=Filtering_of_Samples Filtering]'''
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* Additional info on '''[http://www.leapwiki.com/mediawiki/index.php?title=Shaking%2C_Heating_of_Samples Mixing of samples]'''
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<br>
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=== Application Publications & Posters ===
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[[image: Info icon.png|40px]]  [[Media:The_Plant_Journal_(2006)_45,_847-856.pdf‎|The Plant Journal (2006) - A high-throughput screen for genes from castor that boost
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hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis]]
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<br>
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=== Videos of PAL ===
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<br>
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[[Image:Movie Icon.png|40px]]
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[http://www.youtube.com/user/LEAPTechnologies#g/u LEAP's PAL Application Videos on YouTube]
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<br>
 +
 +
----
 +
{{logo}}
 +
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
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Keywords: plant extract, sample prep, headspace syringe for liquid transfer, heating, shaking
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=== Contact LEAP ===
 +
{{contact|topic=the PAL and the application}}
 +
[[Category:Chronos]]
 +
[[Category:Application_Solutions]]
 +
[[Category:Application Publications & Posters]]

Current revision

Plant Extract
Application Type
  Sample Prep and Inject
Application ID
  Plant1001
Description
  Analyzing a Plant Extract for Chemicals
 ↳ leaptec.com  ↳ leapwiki.com
 ↳ leaptec.com
 ↳ leapwiki.com

Contents

Overview

Plant material and biomass in general are not just for food and feed any longer. They have become the feedstock for fuel, neutraceuticals, drugs and food supplements such as OMEGA-3 or other strong antioxidants. There is a growing research community that looks from a genetics and maximum yield perspective at many bio-organism. A primary example are seeds for oil or algae for OMEGA-3. All these examples have one complication in common: Before analysis for the presence of certain chemicals (molecules) an extract has to be generated out of the raw sample. Fibers, skin and other natural tissue has to be filtered and many constituants of the plants that are of interest have to be extracted in an extraction and separation process. There are many procedures that use a manual approach. As long as the methodology of plant research is its trial and error stage automation can't be justified. However, once a systematic work flow is well defined an automated process is not only more productive but leads to more consistency and less wasteful use of materials, disposables, solvents and of course highly educated personnel's time. LEAP Technologies had many customers that successfully got their extractions automated. Some started out with Seeds, Algae, Tobacco and other leafy plants. The automation was in many cases "just-in time" sample preparation on LEAP's primary XYZ platform the CTC PAL with a subsequent injection onto a Gas Chromatograph or Mass Spectrometer.

Often a GC Twin PAL accomplishes the extraction and filtration before a GC analyzes plant extract material. The application is to take a vial containing plant material, add solvent, heat & agitator, filtering, and then analyze on a 7890 GC. This application may be applicable to any customer who wants to do filtration providing the liquid can be pushed thru the filter plate with a low pressure inert gas through the headspace syringe.

Additional accessories such as an integrated balance and a barcode scanner have been added in the past.

Markets Served:
Elements of this application can be applied to labs requiring any type of filtration of a sample while maximizing throughput.

  • University
  • Research
  • FDA
  • EPA
  • USDA


What is a Plant Extract experiment?
The sample prep procedure is quite simple with the exception of the final sample needing to not contain any of the plant material when it gets to the analysis procedure. The samples are incubated in a heated agitator, and then allowed to settle. The sample solvent, possible containing small pieces of plant material, is filtered. LEAP has a variety of ways to filter whether it is thru a 96 well filter plate or with Uni-Prep Vials. See filtering of samples for the different approaches.


LEAPS Approach
The GC Twin PAL in combination with the Chronos Mastersoftware makes a very effective and flexible workstation. We were able to design and provide a solution which met the following challenges from the customer:

  1. Provide the necessary filtration to have clean samples to analyze. In order to get good blow-out of the liquid through the filter, it was determined that the filter plate should be sealed and have a small nozzle on the underside to ensure accurate collection without crossover to adjacent collection vials.
  2. Throughput. By using a Twin PAL we are able to take advantage of having one PAL do the preparation while the other does the sample injections.
  3. Spatial concerns. Provide a way to get everything that was required to fit.


Photos

Automation of Plant Extract before Gas Chromatography

Seed Crusher for hand use

LEAP Twin PAL with Agitator, 96 well plate filtration, large volume solvent,

Filtration with 96 well plate

LEAP X-Press option on LEAP CTC PAL

UniPrep Filter Diagram

Dilutor with DISPTool

Dilutor on PAL

Chronos software schedule



Application Publications & Posters

The Plant Journal (2006) - A high-throughput screen for genes from castor that boost hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis

Videos of PAL


LEAP's PAL Application Videos on YouTube

 ↳ leaptec.com  ↳ leapwiki.com
 ↳ leaptec.com
 ↳ leapwiki.com

LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.

Contact LEAP

Contact LEAP

For additional information about the PAL and the application, please contact LEAP Technologies.

Retrieved from "http://leapwiki.com/mediawiki/index.php/Prepare_and_Analyze_a_Plant_Extract_by_GC"