On-line SPE LC/MS/MS to Detect Organonitrogen and Triazine Pesticides in Drinking Water
From LEAP
On-line Solid Phase Extraction (SPE) coupled to Liquid Chromatography with tandem Mass Spectrometry (LC/MS/MS) to detect organonitrogen and triazine pesticides at concentrations of 10ng/L in drinking water.
Automated SPE sample clean-up and pre-concentration step utilizing large volume injection (5mL of untreated water samples) prior to LC/MS/MS analysis. With this approach, all pesticides are first trapped on an SPE cartridge and then back flushed onto a reverse phase LC column. The pesticides are then separated on the analytical column using a binary LC gradient and are determined on the ABI LC/MS/MS System.
5mL of tap water were loaded onto a on-line SPE cartridge using a CTC HTC PAL autosampler (fitted with a 5mL syringe, a 5mL loop and a 32 position 10mL sample rack). The trap cartridge was fitted into load position A of a high pressure, PAL 10 port switching valve. An isocratic pump was used to load the sample at 3mL/min onto the trapping cartridge. After 2.5 minutes the valve was switched to position B where an Binary pump was used to provide the gradient elution. After 14.5 minutes the switching valve was switched back to the loading position to analyze the next sample.
ABI Application note for On-Line SPE LC/MS/MS of Pesticides
Useful links
Appliedbiosystems.com Application Note [1]
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [2]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [3]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [4]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [5]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [6]
- Summary of LC Multi Valve Applications [7]
- LEAP Shell Software and application for custom injections and scheduling [8]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
