High Throughput Sample Analysis using Multiple Column LC/MS/MS and Automated Method Optimization Techniques
From LEAP
A 96 well plate, containing 8 different compounds and a simulated PK sample set for each compound were analyzed. Automaton[[1]] software was used to automatically create and optimize the MS/MS methods for each compound, and then execute the quantitative batch for each compound. Working together with Automaton, is Two-Stream Batch[[2]], a software program that allows two columns to be used for the analysis, ultimately leading to higher throughput for the overall analysis.
The PAL will inject the samples alternately into two LC streams at a specified time schedule and the mass spectrometer will acquire data for only a small section of the LC run for each sample. In this example all 8 compounds can be eluted over a 5 minute gradient analysis on one column. The time before and after compound elution can be saved through the multiple LC stream analysis method by selecting a 2.5 minute window.
Poster Number TH-074 from Applied Biosystems/ MDS Sciex ASMS 2003
Media:Highthroughput_Sample_Analysis_using_multiple_columns_ABI.pdf
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