Category:How to

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(Switching the Valve)
 
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and a reminder for infrequent users.
and a reminder for infrequent users.
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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{{logo}}
=== LEAP Syringe Replacement ===
=== LEAP Syringe Replacement ===
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* Prime the replacement syringe manually. Use fresh (dry) solvent, and remove all bubbles.  
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[[image:handheld.png‎|300px|right|PAL Handheld]]
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* On the LEAP Handheld, hit Menu (F1),  
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''' How to Change the Syringe on the PAL '''
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* Chang Syr (F1) and wait for the Z-arm and syringe to position itself
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*   Prime the replacement syringe manually. Use fresh (dry) solvent, and remove all bubbles.  
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*   Place the syringe in the appropriate syringe adapter. Pull the plunger out to approximately 20% of its length
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On the LEAP Handheld, hit Menu (F1),  
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*   Chang Syr (F1) the Injection Unit will move to a location that will facilitate installation of the syringe
*  On the autosampler syringe arm, slide up the clear plastic cover.  
*  On the autosampler syringe arm, slide up the clear plastic cover.  
*  Near the top of the syringe, loosen the red knob by turning it to the right.  
*  Near the top of the syringe, loosen the red knob by turning it to the right.  
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*  At the midpoint of the syringe, rotate the small, black arm by 90 degrees to restrain the syringe body.
 
*  Pinch together the pair of black needle guides that the needle slides through in normal use.  
*  Pinch together the pair of black needle guides that the needle slides through in normal use.  
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*  With the needle guides pinched together, remove the syringe by gently tipping it forward and up.
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*  With the needle guides pinched together, move the syringe(installed in the syringe adapter) partially into the Injection Unit. Guide the needle into the needle guides  
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*  Put into place the new syringe, again pinching together the black needle guides.
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Place the plunger button into the plunger holder. Allow the syringe adapter to "click" into place by magnetic force, against the syringe carrier.  
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Be sure the syringe is against the mounting plate at its top and bottom.  
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*  Rotate the small, black arm back to its original position.  
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*  Tighten the red knob against the syringe plunger by turning the knob to the left.  
*  Tighten the red knob against the syringe plunger by turning the knob to the left.  
*  Slide closed the clear plastic cover.  
*  Slide closed the clear plastic cover.  
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* On the LEAP Handheld, hit the ESC key (repeatedly if needed) to return to the top of the menu.  
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*   On the LEAP Handheld, Press "Home" The plunger moves down until it hits the mechanical stop. This position is stored as the syringes zero volume position. Then the Injection Unit returns to the HOME position.
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* '' After replacing a syringe, always check the needle penetration in the LC valve ''
[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_Change_Syringe.html Changing the Syringe of the PAL ]
[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_Change_Syringe.html Changing the Syringe of the PAL ]
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<br>
<br>
[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_Manual_Injection.html Manual Injection of the PAL ]
[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_Manual_Injection.html Manual Injection of the PAL ]
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<br>
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
----
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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{{logo}}
=== Cleaning the LEAP Syringe with Fast Wash Station ===
=== Cleaning the LEAP Syringe with Fast Wash Station ===
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This can clean the needle or help remove a bubble
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[[image:handheld.png‎|300px|right|PAL Handheld]]
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''' How to clean the syringe at the Fast Wash Station on the PAL '''<br>
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This can clean the needle or help remove a bubble from the syringe
* On the LEAP Handheld, hit Menu (F1)
* On the LEAP Handheld, hit Menu (F1)
*  Clean Syr (F2) <enter>  
*  Clean Syr (F2) <enter>  
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This option will move the LEAP syringe to the desired wash station and will rinse the full length of the syringe body. For new, or completely dry syringes, it is recommended to slightly pre-wet the syringe with solvent to displace some of the air.
This option will move the LEAP syringe to the desired wash station and will rinse the full length of the syringe body. For new, or completely dry syringes, it is recommended to slightly pre-wet the syringe with solvent to displace some of the air.
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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{{logo}}
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=== Rinsing the Wash Station and Tubing with Solvent after refilling Solvent Reservoir ===
=== Rinsing the Wash Station and Tubing with Solvent after refilling Solvent Reservoir ===
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[[image:handheld.png‎|300px|right|PAL Handheld]]
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''' How to rinse the fast wash station solvent reservoirs '''
* To replenish the Wash solvent, close the stopcock at the base of the Wash Bottle  
* To replenish the Wash solvent, close the stopcock at the base of the Wash Bottle  
* Disconnect the tube at the black nut that goes into the white stopcock at the base of the bottle  
* Disconnect the tube at the black nut that goes into the white stopcock at the base of the bottle  
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*  When reads Deact Valve, the solenoid is open  
*  When reads Deact Valve, the solenoid is open  
* Hit the ESC key (repeatedly if needed) to return to the top of the menu.  
* Hit the ESC key (repeatedly if needed) to return to the top of the menu.  
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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{{logo}}
=== Needle Penetration Adjustment for Injector Port ===  
=== Needle Penetration Adjustment for Injector Port ===  
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[[image:handheld.png‎|300px|right|PAL Handheld]]
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''' Adjusting the Needle Penetration of the Injector '''
* On the LEAP Handheld, hit Menu (F1)
* On the LEAP Handheld, hit Menu (F1)
*  Setup, <enter>
*  Setup, <enter>
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* When adjusted, hit Movto Zero  
* When adjusted, hit Movto Zero  
* Hit the ESC key (repeatedly if needed) to return to the top of the menu.  
* Hit the ESC key (repeatedly if needed) to return to the top of the menu.  
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<br>
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[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_Injection_Depth.html Changing the Injection Depth at Valve of the PAL ]
[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_Injection_Depth.html Changing the Injection Depth at Valve of the PAL ]
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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{{logo}}
=== Switching the Valve ===
=== Switching the Valve ===
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[[image:handheld.png‎|300px|right|PAL Handheld]]
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''' How to activate(toggle) the valve '''
* On the LEAP Handheld, hit Menu (F1)
* On the LEAP Handheld, hit Menu (F1)
*  Utilities <enter>  
*  Utilities <enter>  
*  Injector <enter> (hit <enter> again to select default injector)  
*  Injector <enter> (hit <enter> again to select default injector)  
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Pressing Act Valve (F2) puts the valve in the red light Off position = injection port connected to loop <br>
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* Pressing Act Valve (F2) puts the valve in the red light Off position = injection port connected to loop ''(for at Type W valve)''<br>
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Pressing Deact Valve (F2) puts the valve in the red light On position = injection port not connected to loop <br>
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* Pressing Deact Valve (F2) puts the valve in the red light On position = injection port not connected to loop ''(for at Type W valve)''<br>
* Hit the ESC key (repeatedly if needed) to return to the top of the menu.  
* Hit the ESC key (repeatedly if needed) to return to the top of the menu.  
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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----
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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{{logo}}
=== Changing Trays ===
=== Changing Trays ===
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[[image:handheld.png‎|300px|right|PAL Handheld]]
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''' How to change the trays on the PAL '''
* On the LEAP Handheld, hit Menu (F1)
* On the LEAP Handheld, hit Menu (F1)
* Setup (F3) <enter>  
* Setup (F3) <enter>  
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* Be sure to save each coordinate  
* Be sure to save each coordinate  
* Check the corners of the Tray  
* Check the corners of the Tray  
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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{{logo}}
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=== Setting Needle Penetration Depth for a Particular Tray ===
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=== Setting Needle Penetration Depth for a Particular Tray Type===
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*Setting the Tray Holder XYZ position (performed at installation & described elsewhere) also enables the Needle Guide to find corner positions of a specified Tray (or well plate)  
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[[image:handheld.png‎|300px|right|PAL Handheld]]
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*This procedure guides a user to select a Tray, then adjust the needle penetration for a vial resting in that Tray. <br>All of the XYZ positions for the Tray Holder, Wash Stations, Injector Port, and Waste should be calibrated prior to this procedure  
+
''' Changing the needle penetration for a specific tray type'''
 +
Setting the Tray Holder XYZ position (performed at installation & described elsewhere) also enables the Needle Guide to find corner positions of a specified Tray (or well plate) <br>
 +
This procedure guides a user to select a Tray, then adjust the needle penetration for a vial resting in that Tray. <br>All of the XYZ positions for the Tray Holder, Wash Stations, Injector Port, and Waste should be calibrated prior to this procedure <br> Please note: only 1 needle penetration is available per tray type and is used for all positions of that tray type <br>
* On the LEAP Handheld, hit Menu (F1)
* On the LEAP Handheld, hit Menu (F1)
*  Utilities <enter>  
*  Utilities <enter>  
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*    Start by entering a Needle Penetr depth of 0.0 mm <enter>  
*    Start by entering a Needle Penetr depth of 0.0 mm <enter>  
*    Movto 001 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter>  
*    Movto 001 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter>  
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*    Movto 009 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter>  
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*    Movto 009 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter> ''(assumes VT-54)''
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*    Movto 054 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter>  
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*    Movto 054 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter> ''(assumes VT-54)''
* Home  
* Home  
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<br>
 
[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_MT96_Tray.html Calibration of 96 well microtiter plate of the PAL ]
[[Image:Movie Icon.png|40px]] [http://www.micronmr.com/download/video/Leap_MT96_Tray.html Calibration of 96 well microtiter plate of the PAL ]
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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This information is not meant to replace on-site familiarization but provide a basic outline of the steps involved. Please refer to your PAL owners manual for detailed information.
This information is not meant to replace on-site familiarization but provide a basic outline of the steps involved. Please refer to your PAL owners manual for detailed information.
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''For some vides you will be leaving the LEAPWiki and going to the MicroNMR Website. To return just press the back button in your browser window.''
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''For some videos you will be leaving the LEAPWiki and going to the MicroNMR Website. To return just press the back button in your browser window.''
The videos are FLASH videos and should be easily viewed within any standard web browser. Simply click on one of the link to play that video. If you are having trouble viewing the videos you may need to install the Flash Plug-in from the Adobe website  (www.adobe.com).
The videos are FLASH videos and should be easily viewed within any standard web browser. Simply click on one of the link to play that video. If you are having trouble viewing the videos you may need to install the Flash Plug-in from the Adobe website  (www.adobe.com).
''There is AUDIO so make sure your speakers are on! ''
''There is AUDIO so make sure your speakers are on! ''
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MicroNMR a partner of LEAP Technologies has put together some basic Training Videos for the PAL robot
MicroNMR a partner of LEAP Technologies has put together some basic Training Videos for the PAL robot
<br>A special thanks to MicroNMR for providing the videos on their website for all to access http://www.micronmr.com/training_videos.html
<br>A special thanks to MicroNMR for providing the videos on their website for all to access http://www.micronmr.com/training_videos.html
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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[[Image:service.png|600px|thumb|center|LEAP Tech Support and Service ]]
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{{logo}}
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
=== Contact LEAP ===
=== Contact LEAP ===
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{{contact|topic=How to with the PAL}}
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[[image:Contact_Info.png‎|40px]] [http://www.leaptec.com/contact-us.php Contact the LEAP office]
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[[Category:Glossary]]
[[Category:Glossary]]
[[Category:Consumables]]
[[Category:Consumables]]
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[[Category:Definitions]]

Current revision

PAL

PAL (commonly referred to as LEAP and CTC) is an X,Y,Z Robot with syringe only concept.
Included here are some basic how to info as a reference to go along with the training and orientation provided on-site or by phone by LEAP Technologies. It is intended to provide an overview for new users and a reminder for infrequent users.

 ↳ leaptec.com  ↳ leapwiki.com

Contents

LEAP Syringe Replacement

PAL Handheld

How to Change the Syringe on the PAL

  • Prime the replacement syringe manually. Use fresh (dry) solvent, and remove all bubbles.
  • Place the syringe in the appropriate syringe adapter. Pull the plunger out to approximately 20% of its length
  • On the LEAP Handheld, hit Menu (F1),
  • Chang Syr (F1) the Injection Unit will move to a location that will facilitate installation of the syringe
  • On the autosampler syringe arm, slide up the clear plastic cover.
  • Near the top of the syringe, loosen the red knob by turning it to the right.
  • Pinch together the pair of black needle guides that the needle slides through in normal use.
  • With the needle guides pinched together, move the syringe(installed in the syringe adapter) partially into the Injection Unit. Guide the needle into the needle guides
  • Place the plunger button into the plunger holder. Allow the syringe adapter to "click" into place by magnetic force, against the syringe carrier.
  • Tighten the red knob against the syringe plunger by turning the knob to the left.
  • Slide closed the clear plastic cover.
  • On the LEAP Handheld, Press "Home" The plunger moves down until it hits the mechanical stop. This position is stored as the syringes zero volume position. Then the Injection Unit returns to the HOME position.
  • After replacing a syringe, always check the needle penetration in the LC valve

Changing the Syringe of the PAL
Changing the Needle Sleeve of the PAL
Manual Injection of the PAL




















 ↳ leaptec.com  ↳ leapwiki.com

Cleaning the LEAP Syringe with Fast Wash Station

PAL Handheld

How to clean the syringe at the Fast Wash Station on the PAL
This can clean the needle or help remove a bubble from the syringe

  • On the LEAP Handheld, hit Menu (F1)
  • Clean Syr (F2) <enter>
  • Select a Wash Station <enter>
  • Enter the number of times the syringe should rinse <enter>
  • On the LEAP Handheld, hit the ESC key (repeatedly if needed) to return to the top of the menu.

This option will move the LEAP syringe to the desired wash station and will rinse the full length of the syringe body. For new, or completely dry syringes, it is recommended to slightly pre-wet the syringe with solvent to displace some of the air.
























 ↳ leaptec.com  ↳ leapwiki.com

Rinsing the Wash Station and Tubing with Solvent after refilling Solvent Reservoir

PAL Handheld

How to rinse the fast wash station solvent reservoirs

  • To replenish the Wash solvent, close the stopcock at the base of the Wash Bottle
  • Disconnect the tube at the black nut that goes into the white stopcock at the base of the bottle
  • Rinse the bottle with fresh solvent and drain some solvent out through the stopcock
  • Place the Wash bottle back into position, re-connect its tube, and open the stopcock
  • On the LEAP Handheld, hit Menu (F1)
  • Utilities <enter>
  • Wash Station <enter>
  • Select desired wash station <enter>
  • Press Act Valve (F2) to open the valve (located in the solenoid under the Wash Station)
  • Rinse the tube until all the bubbles in it are flushed out (several seconds)
  • Press Deact Valve (F2) to close the solenoid valve
  • When F2 reads Act Valve, the solenoid is closed
  • When reads Deact Valve, the solenoid is open
  • Hit the ESC key (repeatedly if needed) to return to the top of the menu.
























 ↳ leaptec.com  ↳ leapwiki.com

Needle Penetration Adjustment for Injector Port

PAL Handheld

Adjusting the Needle Penetration of the Injector

  • On the LEAP Handheld, hit Menu (F1)
  • Setup, <enter>
  • Objects, <enter>
  • Injectors, <enter>
  • LC V1v1, Z, <enter>, and set Z to 0 mm, <enter>
  • Needle Penetr(ation), and set Needle Penetr to 0 mm or less, <enter>
  • Hit Check Pos(ition) F1
  • Choose Z, <enter>, and adjust it using the black needle guide as reference; save with <enter>
  • Choose Needle Penetr(ation), <enter>, adjust to clunk, and back up 0.2 mm, <enter>
  • Verify the Needle Penetr setting by
  • Select Needle Penetr, <enter>, and listen for thunk; it might thunk a little, but not as badly. If it thunks badly, readjust the Needle Penetr till it thunks back up an additional 0.2 mm, and hit <enter>.
  • Repeat this step as needed. It often takes several adjustments, but is then consistent.
  • When adjusted, hit Movto Zero
  • Hit the ESC key (repeatedly if needed) to return to the top of the menu.

Changing the Injection Depth at Valve of the PAL





















 ↳ leaptec.com  ↳ leapwiki.com

Switching the Valve

PAL Handheld

How to activate(toggle) the valve

  • On the LEAP Handheld, hit Menu (F1)
  • Utilities <enter>
  • Injector <enter> (hit <enter> again to select default injector)
  • Pressing Act Valve (F2) puts the valve in the red light Off position = injection port connected to loop (for at Type W valve)
  • Pressing Deact Valve (F2) puts the valve in the red light On position = injection port not connected to loop (for at Type W valve)
  • Hit the ESC key (repeatedly if needed) to return to the top of the menu.























 ↳ leaptec.com  ↳ leapwiki.com

Changing Trays

PAL Handheld

How to change the trays on the PAL

  • On the LEAP Handheld, hit Menu (F1)
  • Setup (F3) <enter>
  • Objects <enter>
  • Trays <enter>
  • Select Desired Tray (usually Tray01, Tray02, Tray….) <enter>
  • Scroll down to highlight ‘Tray Type’ <enter> and scroll through options
  • VT54 – Vial Tray with 54 locations (blue)
  • MT96 – Microplate Tray with 96 wells (short)
  • DW96 – Deep Well tray with 96 wells (tall)
  • Confirm/Adjust XYZ to coincide with Tray position 1 and well 1 (see detailed directions below)
  • Be sure to save each coordinate
  • Check the corners of the Tray





















 ↳ leaptec.com  ↳ leapwiki.com

Setting Needle Penetration Depth for a Particular Tray Type

PAL Handheld

Changing the needle penetration for a specific tray type Setting the Tray Holder XYZ position (performed at installation & described elsewhere) also enables the Needle Guide to find corner positions of a specified Tray (or well plate)
This procedure guides a user to select a Tray, then adjust the needle penetration for a vial resting in that Tray.
All of the XYZ positions for the Tray Holder, Wash Stations, Injector Port, and Waste should be calibrated prior to this procedure
Please note: only 1 needle penetration is available per tray type and is used for all positions of that tray type

  • On the LEAP Handheld, hit Menu (F1)
  • Utilities <enter>
  • Tray (select Tray – Tray## or Stack - Stk1-##) <enter>
  • Needle Penetr <enter>
  • Start by entering a Needle Penetr depth of 0.0 mm <enter>
  • Movto 001 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter>
  • Movto 009 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter> (assumes VT-54)
  • Movto 054 (F3), Needle Penetr <enter>, Dial depth downward till downward syringe motion halts, back up 0.1 mm, <enter> (assumes VT-54)
  • Home

Calibration of 96 well microtiter plate of the PAL



















This information is not meant to replace on-site familiarization but provide a basic outline of the steps involved. Please refer to your PAL owners manual for detailed information.

LEAP offers installation and familiarization training with every new system. The twelve month warranty includes unlimited telephone support and module exchange programs. Extended warranties include annual preventative maintenance in addition to telephone support and module exchange programs.

For customers that want to write their own custom macros and injection cycles, LEAP offers software training at our facility in Carrboro, NC.



For some videos you will be leaving the LEAPWiki and going to the MicroNMR Website. To return just press the back button in your browser window. The videos are FLASH videos and should be easily viewed within any standard web browser. Simply click on one of the link to play that video. If you are having trouble viewing the videos you may need to install the Flash Plug-in from the Adobe website (www.adobe.com). There is AUDIO so make sure your speakers are on!

MicroNMR a partner of LEAP Technologies has put together some basic Training Videos for the PAL robot
A special thanks to MicroNMR for providing the videos on their website for all to access http://www.micronmr.com/training_videos.html


LEAP Tech Support and Service
LEAP Tech Support and Service

 ↳ leaptec.com  ↳ leapwiki.com

LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.

Contact LEAP

Contact LEAP

For additional information about How to with the PAL, please contact LEAP Technologies.

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Pages in category "How to"

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