Category:DBS
From LEAP
| DBS SCAP PAL |
| Application Type | |
| DBS PAL | |
| Application ID | |
| DBS PAL | |
| Description | |
| Robotic Automated System for automating DBS processing with the PAL Robot |
Automated sample extraction platform for online LC-MS/MS bioanalysis
Contents |
As Seen at the CPSA October 2010
LEAP Technologies presented "Novel Walk Away Automation for DBS sample extraction from 4-Spot Cards to LC-MS" at the CPSA 2010 annual meeting held in Pennsylvania.
The Dry Blood Spot (DBS) technique of eluding the blood sample from filter paper cards still lacks simple, reliable and inexpensive automation until today with LEAP Technologies and the PAL Platform. There are approximately 100 methods/applications known for these sample carrier DBS cards with many more being developed everyday. In many such areas automation will be a tremendous benefit. It will not only be labor saving, but also decrease handling, which means less errors and more consistency in getting the samples introduced into a separation and then the analytical system.There are many benefits of the DBS approach for the bioanalyst, both ethical and financial, the use of DBS involves a reconsideration of current workflows such as on-line extractions.
The work flow of the automation approach step by step was discussed. The experience with hardware and software so far was be reviewed, including experience with the built-in internal standard addition. The SCAP DBS has patent pending technology applied and is simple to use. The approach of eluting the dry blood sample off the card directly to a multi-column system by-passes all manual and cumbersome treatment steps of the sample cards. First results from needed cycle times and throughput were presented. This is LEAP's latest approach to automated DBS using the PAL platform.
Novel Walk Away Automation for DBS Sample Extraction from 4-Spot Cards to LC-MS Authors: Brian Peat, Peter Smith, Dave Holub, LEAP Technologies, Inc, PO Box 969, Carrboro, NC 27510
Background
The Dry Blood Spot (DBS) technique has a long history in various areas of whole blood sample collection, preservation, transportation and laboratory analysis. It has been applied where very little blood sample is available for many different reasons. As its popularity increased during the last few years in new areas such as pre-clinical drug discovery research, the call for automation in the laboratory also increased. The use of the low sample volumes allows pediatric sampling and improves study recruitment. DBS reduces cost in animals, procedures and test substances. DBS cards can be stored at ambient conditions, reducing sample storage and transport costs.
The automated method on the SCAP (Sample Card & Prep) platform applies a direct elution process from the DBS card to the LC or LC/MS system. An automatic internal standard addition during the elution process solidifies the consistency of results. The Robotic Platform is a well proven XYZ system (LEAP PAL) that is used in many configurations in research settings, production and quality assurance situations. The workflow is simple and can be easily observed on the SCAP PAL. A gripper for card handling, card racks, a clamp-elution station and the necessary valving system are the key components of the system. There is no syringe needed for sample injections and there is no injection valve. The sample plus the added internal standard are coming off the filter paper card to a pre-column by means of an LC loading pump. The gradient pump will elude the trapped sample analytes off the pre-column onto a normal LC or UHPLC system with UV or MS detector. The clamp device can have different diameter dyes, allowing small to large diameter elution spots on the card. This has proven to be a useful variable to eliminate possible dilution steps when concentrations of analytes are relatively high.
Automation no longer has just the value of labor cost savings. It is clearly recognized for error reduction and more consistent results. Manual sample preparation has always raised questions of consistency. Technology often simplifies the process and manual intervention. In this case several steps of the sample prep process are eliminated and escape a complex automation task. These are punching out filter paper samples from the original carrier as well as the elution process in a solvent vial. Eliminating the elution process from the filter paper as an intermediary step avoids unnecessary dilution, inconsistency, and contamination normally possible during a manual handling process.
This SCAP PAL makes the DBS process in the laboratory a hands off process. The cycle time is short and the analysis time is more likely the throughput limiting factor. Even though field experience is still limited, the deployment of the PAL platform into this emerging application area provides proven reliable, simple automation for higher consistency and less sample mix-up and contamination. The Dry Blood Spot (DBS) technique of eluding the blood sample from filter paper cards still lacks simple, reliable and inexpensive automation. Recently, Prolab Instruments, GmbH introduced a system (SCAP DBS System) built on the popular PAL XYZ robotic platform. LEAP Technologies has teamed up with the system developer Prolab to offer the SCAP DBS system in the U.S. and Canada. There are approximately 100 methods and applications known for these sample carrier DBS cards. In many such areas automation would be a tremendous benefit. It would not only be labor saving, but also decrease handling, which means less errors and more consistency in getting the samples introduced into a separation and then the analytical system. The presentation goes through the work flow of the automation approach step by step. The experience with hardware and software so far will be reviewed, including experience with the built-in internal standard addition. The SCAP DBS has patent pending technology applied and is simple to use. The approach of eluting the dry blood sample off the card directly to a multi-column system by-passing all manual and cumbersome treatment steps of the sample cards
Some advantages of using DBS:
- Collection simple
- Most analytes stable
- Filter paper is an ideal method for collecting whole blood
- Ease of transport - no refrigeration or dry ice required
- Storage easy and compact
- Safety and handling exposure
- Centralized technology and laboratory (reference labs can easily monitor remote site performance)
Overview
Automated Sample Processing of DBS Card
The dried blood spot (DBS) technology coupled to highly sensitive LC-MS/MS systems opens up new ways in bioanalytics both in the health care and the pharmaceutical industry.
In the health care industry, complete measurements are done to diagnose patients to determine the efficacy of administrated drugs or to screen for early detection of desires. In the pharmaceutical industry, measurements are done during the development process of new drugs (e.g. PK/TK studies, etc.)The major drawback of conventional DBS bio- analysis is the time consuming, error prone and labor intensive nature of manual sample pre-treatment. The SCAP DBS system represents a unique approach for direct and automated DBS analysis and eliminates the drawback of current methods.
The SCAP DBS system fully automates the DBS cards handling and allows online sample extraction without the need for punching of DBS cards.
Advantages of LEAP's automation:
- Fully automated SCAP DBS system sample card handling for LC/MS-MS analysis
- Optimized extraction of DBS
- Simplified method development by online extraction
- Fully automated process saves time and labor costs
- Sample Preparation is no longer the rate limiting factor
- No need for additional consumables
SCAP DBS card sample automation process:
The DBS rack with the DBS cards is placed into the SCAP DBS system. According to the sample queue the robotic arm transfers the card into the clamp module where the DBS card is integrated into the HPLC flow path for online extraction without punching of DBS cards. The different adapters available for the SCAP DBS system allow extracting different areas of the DBS and seal the DBS card by applying up to 200 bars. The valve system configuration allows the on- line addition of internal standard (ISTD) during sample extraction. A SPE pre column is used for online sample cleanup prior to analytical separation on the main column.
Automated sample extraction platform for online LC-MS/MS bioanalysis LEAP PAL Configurations use the following LEAP CTC Analytics PAL's are required to operate a SCAP DBS system:
PAL HTC9-xt PAL50 instrument (one DBS dual tray)
PAL HTS9-xt PAL80 instrument (three DBS dual trays)
PAL HTX9-xt PAL120 instrument (five DBS dual trays)
PAL 1, 2, 3 Valve Drive (Serial Valve drives) with six port or ten port valve (depending on your application)
Cycle composer with macro editor - Other software under review
Photos
 LEAP CTC SCAP DBS Extraction Workstation using the PAL |
 LEAP CTC SCAP DBS clamp adapters using the PAL |
 LEAP CTC SCAP DBS clamp using the PAL |
 LEAP CTC SCAP DBS gripper using the PAL |
 LEAP CTC SCAP DBS rack using the PAL |
 LEAP CTC SCAP DBS tray using the PAL |
 LEAP CTC SCAP DBS valve using the PAL |
 LEAP Prolab SCAP AUTOMATEDBS using the PAL x,y,z Robot |
Other Reference Material
Novel Walk Away Automation for DBS sample extraction from 4-Spot Cards to LC-MS
Accessories for the PAL
Other Accessories for PAL Robots
Videos of the PAL
LEAP's PAL Application Videos on YouTube
LEAP Technologies provides fully automated sample processing workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. Including the Automated sample extraction platform for online LC-MS/MS bioanalysis. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP also offers process control systems and stand alone workstations. LEAP is an open, neutral resource for technical support of existing PAL. Keep your PAL in peak working order LEAP offers full support and service for the PAL platform with over 11 years of history with the PAL. LEAP can also write custom macros, cycles, and scheduling software for your specific applications. We're proud that LEAP is known as a productivity improver for laboratories including government labs in agencies such as FDA, USDA, EPA, FBI, Armed Forces, Homeland Security and others function more efficiently. Robotic sample prep equipment works well in government labs and challenges lab technicians to keep feeding those more samples. Analytical equipment such as chromatographs and mass spectrometers are expensive devices and also expensive to maintain. LEAP can help to keep them running and analyze samples around the clock. LEAP has most of its laboratory automation robots under GSAcontract and is eager to service and maintain everything it sells to keep your expensive lab equipment running around the clock. CTC Analytics Combi PAL and HTS PAL for Front-End Automation for LC and GC applications. LEAP offers customized on-site training. LEAP Is continuing to develop FAQ, Solutions, Tips & Techniques,a Learning Center with the LEAP Wiki for the benefit of all PAL Platform Robotic users. We will continue exceeding customer expectations for quality, cost effective and efficient results using the most current technology and expertise in our industry. Our aim is to be the ultimate knowledge base on everything related to the PAL. LEAP is known as the autosamplerguys being in business over twenty years. LEAP has extensive multi-vendor experience with integrating the PAL. HTX, HTS, HTC, HTX-xt, HTS-xt, HTC-xt, LC-Mini, Combi PAL,COMBI-xt PAL, and GC PAL models. We have the Quality, Training, Parts availability, Infrastructure, and Experience.LEAP is sometimes referred to as an Application Automation House who is continually adding new products and solutions to its Lab Automation line. As we do so we change the content of this web site so please check back.
Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions whether it is our standard applications or customized to your needs. The Combi-xt PAL autosampler is able to perform liquid and headspace analysis. Headspace options include Solid Phase Micro-extraction (SPME) and ITEX-2 as alternative to purge and trap. The HTS-xt PAL autosampler dramatically increases sample throughput by offering automated sample prep, Peltier cooled and heated sample storage using the cooled stack, Peltier cooled and heated sample storage using the cooled stack and low carryover due to the self-cleaning DLW (Dynamic Load and Wash) module and L-Mark syringes, peltier cooled valves and columns. LEAP provides automation that fits your needs and reduces errors, increases sample capacity, increases efficiency, reduces cost by supplying long term productivity improvements with user friendly software and flexible hardware. LEAP offers full support of integrated and non-integrated PAL software with both sales and support for Cruise Control, Cycle Composer and Cycle Composer Macro Editor, Agilent ChemStation CTC Control, Mass Hunter and EZChrom CTC integration, Xcalibur, Waters Masslynx or Analyst CTC Control software, Thermo (Finnegan) XCalibur, LEAP Shell, and Chronos software. The PAL is more than just an autosampler, it is a high-quality and efficient laboratory robot platform that can be customized by LEAP Technologies to meet your specific needs.
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For additional information about LEAP and the PAL Platform, please contact LEAP Technologies. |
The information and references contained in this web site are believed to be accurate and the most current information available.
As Seen at AAPS 2010 in New Orleans Booth
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