Category:DBS
From LEAP
| DBS SCAP PAL |
| Application Type | |
| DBS PAL | |
| Application ID | |
| DBS PAL | |
| Description | |
| Robotic Automated System for automating DBS processing with the PAL Robot |
Automated DBS Extraction Station
Contents |
Overview
Automated Sample Processing of BBS Card
The dried blood spot (DBS) technology coupled to highly sensitive LC-MS/MS systems opens up new ways in bioanalytics both in the healthcare and the pharmaceutical industry.
In the healthcare industry, complete mea- surements are done to diagnose patients to determine the efficacy of administrated drugs or to screen for early detection of desires. In the pharmaceutical industry, measurements are done during the development process of new drugs (e.g. PK/TK studies, etc.)The major drawback of conventional DBS bio- analysis is the time consuming, error prone and labor intensive nature of manual sample pre-treatment. The SCAP DBS system represents a unique approach for direct and automated DBS analysis and eliminates the drawback of current methods.
The SCAP DBS system fully automates the DBS cards handling and allows online sample extraction without the need for punching of DBS cards.
Advantages:
- Fully automated SCAP DBS system sample card handling for LC/MS-MS analysis
- Optimized extraction of DBS
- Simplified method development by online extraction
- Fully automated process saves time and labor costs
- No need for additional consumables
SCAP DBS sample process:
The DBS rack with the DBS cards is placed into the SCAP DBS system. According to the sample queue the robotic arm transfers the card into the clamp module where the DBS card is integrated into the HPLC flow path for online extraction without punching of DBS cards. The different adapters available for the SCAP DBS system allow extracting different areas of the DBS and seal the DBS card by applying up to 200 bars. The valve system configuration allows the on- line addition of internal standard (ISTD) during sample extraction. A SPE pre column is used for online sample cleanup prior to analytical separation on the main column.
SCAP PAL Configuration
The following LEAP CTC Analytics SCAP PAL components are required to operate a SCAP DBS system
PAL HTC9-xt PAL50 instrument (one DBS dual tray) PAL HTS9-xt PAL80 instrument (three DBS dual trays) PAL HTX9-xt PAL120 instrument (five DBS dual trays)
PAL 1, 2, 3 Valve Drive (Serial Valve drives) with six port or ten port valve (depending on your application)
Cycle composer with macro editor - Other software under review
Photos
LEAP CTC SCAP DBS Extraction Workstation using the PAL |
 LEAP CTC SCAP DBS clamp adapters using the PAL |
 LEAP CTC SCAP DBS clamp using the PAL |
 LEAP CTC SCAP DBS gripper using the PAL |
 LEAP CTC SCAP DBS gripper using the PAL |
 LEAP CTC SCAP DBS rack using the PAL |
 LEAP CTC SCAP DBS tray using the PAL |
 LEAP CTC SCAP DBS valve using the PAL |
 LEAP CTC SCAP DBS valve using the PAL |
Other Reference Material
LEAP Weighing Station Information Document
Accessories for the PAL
Other Accessories for PAL Robots
Videos of the PAL
LEAP's PAL Application Videos on YouTube
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications.Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
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As Seen at the CPSA October 2010
LEAP Technologies will present "Novel Walk Away Automation for DBS sample extraction from 4-Spot Cards to LC-MS" at the CPSA 2010 annual meeting being held at the Bucks County Sheraton Hotel, 400 Oxford Valley Road, Langhorne, Pennsylvania 19047.
To see information about this Dried Blood Spot system please visit us on Tuesday, October 19th from 1 - 3:30pm at our Poster.
The Dry Blood Spot (DBS) technique of eluding the blood sample from filter paper cards still lacks simple, reliable and inexpensive automation until today with LEAP Technologies and the PAL Platform. There are approx.100 methods/applications known for these sample carrier DBS cards. In many such areas automation will be a tremendous benefit. It will not only be labor saving, but also decrease handling, which means less errors and more consistency in getting the samples introduced into a separation and then the analytical system.
The poster goes through the work flow of the automation approach step by step. The experience with hardware and software so far will be reviewed, including experience with the built-in internal standard addition. The SCAP DBS has patent pending technology applied and is simple to use. The approach of eluting the dry blood sample off the card directly to a multi-column system by-passes all manual and cumbersome treatment steps of the sample cards. First results from needed cycle times and throughput will be presented. This is LEAP's approach to automated dbs using the PAL platform.
Beta test results with analytical data compared to the traditional manual method will also be shown. We look forward to sharing this exciting new technique with you.
LEAP Technologies provides fully automated sample processing workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP also offers process control systems and stand alone workstations. LEAP is an open, neutral resource for technical support of existing PAL. Keep your PAL in peak working order LEAP offers full support and service for the PAL platform with over 11 years of history with the PAL. LEAP can also write custom macros, cycles, and scheduling software for your specific applications. We're proud that LEAP is known as a productivity improver for laboratories including government labs in agencies such as FDA, USDA, EPA, FBI, Armed Forces, Homeland Security and others function more efficiently. Robotic sample prep equipment works well in government labs and challenges lab technicians to keep feeding those more samples. Analytical equipment such as chromatographs and mass spectrometers are expensive devices and also expensive to maintain. LEAP can help to keep them running and analyze samples around the clock. LEAP has most of its laboratory automation robots under GSAcontract and is eager to service and maintain everything it sells to keep your expensive lab equipment running around the clock. CTC Analytics Combi PAL and HTS PAL for Front-End Automation for LC and GC applications. LEAP offers customized on-site training. LEAP Is continuing to develop FAQ, Solutions, Tips & Techniques,a Learning Center with the LEAP Wiki for the benefit of all PAL Platform Robotic users. We will continue exceeding customer expectations for quality, cost effective and efficient results using the most current technology and expertise in our industry. Our aim is to be the ultimate knowledge base on everything related to the PAL. LEAP is known as the autosamplerguys being in business over twenty years. LEAP has extensive multi-vendor experience with integrating the PAL. HTX, HTS, HTC, HTX-xt, HTS-xt, HTC-xt, LC-Mini, Combi PAL,COMBI-xt PAL, and GC PAL models. We have the Quality, Training, Parts availability, Infrastructure, and Experience.LEAP is sometimes referred to as an Application Automation House who is continually adding new products and solutions to its Lab Automation line. As we do so we change the content of this web site so please check back.
Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions whether it is our standard applications or customized to your needs. The Combi-xt PAL autosampler is able to perform liquid and headspace analysis. Headspace options include Solid Phase Micro-extraction (SPME) and ITEX-2 as alternative to purge and trap. The HTS-xt PAL autosampler dramatically increases sample throughput by offering automated sample prep, Peltier cooled and heated sample storage using the cooled stack, Peltier cooled and heated sample storage using the cooled stack and low carryover due to the self-cleaning DLW (Dynamic Load and Wash) module and L-Mark syringes, peltier cooled valves and columns. LEAP provides automation that fits your needs and reduces errors, increases sample capacity, increases efficiency, reduces cost by supplying long term productivity improvements with user friendly software and flexible hardware. LEAP offers full support of integrated and non-integrated PAL software with both sales and support for Cruise Control, Cycle Composer and Cycle Composer Macro Editor, Agilent ChemStation CTC Control, Mass Hunter and EZChrom CTC integration, Xcalibur, Waters Masslynx or Analyst CTC Control software, Thermo (Finnegan) XCalibur, LEAP Shell, and Chronos software. The PAL is more than just an autosampler, it is a high-quality and efficient laboratory robot platform that can be customized by LEAP Technologies to meet your specific needs.
Contact LEAP
 Contact LEAP
For additional information about LEAP and the PAL Platform, please contact LEAP Technologies. |
The information and references contained in this web site are believed to be accurate and the most current information available.
Keywords: SCAP, Dried Blood Spot,DBS, online extraction, automated sample extraction platform for online LC-MS/MS bioanalysis, sample card, automated, placement, DBS integrated sample processing, superior alternative, fully automated, optimized extraction of DBS, simplified method development by online extraction, fully automated process saves time and labor costs, no need for additional consumables or centrifuge / filtration, PAL, multiple clamp adapters, LEAP, CTC, HTS, HTS, HTX, Drug metabolism (DM), pharmacokinetic (PK) and toxicokinetic (TK) studies, DMPK, process DBS samples rapidly and with great precision using robotic systems specially developed for the purpose, practical solution to processing after with shipping and storing blood samples that require controlled handling and frozen transportation and storage, systems that can prepare and process large quantities of Dried Blood Spot (DBS) samples for LC/MS/MS analysis, Dried Blood Spots Replacing Plasma Samples,new flow through concept that allows for the direct analysis of sample spots from paper into the mass spectrometer via online solid-phase extraction, The dried blood spot (DBS) method is an alternative technique that overcomes traditional drawbacks like (manual punching and extracting, Dried blood spots (DBS) have been used for years for HIV-1 RNA, DNA, Genotyping, and other diagnostic assays, increase in sensitivity is obtained using this direct flow through elution technique, compared to the punch and elute method, Dried blood spots can be used as an easy and inexpensive means for the collection and storage of peripheral blood specimens from infants, children and adults in settings where collection and storage of plasma is not optimal. Dried blood spots use a small amount of peripheral blood on protein saver filter paper cards from infant heel pricks, or finger sticks from adults and require less skill and supplies than venous blood draws, automation after elution of the sample off a dry card format with an autosampler, the cost and difficulties of cold chain shipping of plasma samples is greatly reduced by the use of dried blood spots and they can be shipped as non-dangerous goods.Widely used to screen for metabolic problems in newborn babies, it is now being successfully employed in PK studies,The use of dried blood spot (DBS) to acquire, ship, store, and test samples is not new. However, it has gained tremendous visibility and is rapidly being adopted in all aspects of bioanalysis. In the near term, it is actually more challenging for bioanalytical scientists. The development of automation for the DBS format and newer technology similar to dried blood spotting has been developed to overcome the challenge of manual punching and extracting, direct elution from card into further solid phase extraction clean-up,Whatman DMPK cards,Flow through DBS extraction device, 4 spot, Dried Blood Spots (DBS) have been show to be a useful means of collecting, storing and shipping blood samples for quantitative drug analysis and provides many advantages over conventional plasma collection, simplify and speed up the process and improve quality of the analysis, automation of dried blood spots in United States, cycle composer, LEAP Technologies
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