MALDI Spotter with the PAL
From LEAP
| MALDI Spotter with the PAL |
| Application Type | |
| STANDARD | |
| Application ID | |
| MALDI Spotter | |
| Description | |
| MALDI Spotter with the PAL |
Contents |
PAL MALDI Option General System Overview
The PAL MALDI Option micro collector/spotter is an add-on PAL module for micro scale fraction collection or MALDI Spotting together with any Capillary LC or Nanoflow LC system. The collection of very low volume fractions requires low delay volumes and high position accuracy of the capillary tip.
The PAL is equipped with the special MALDI Spotting attachment which is configured as show below. The tool holds the outflow of the Nano LC capillary tubing and when picked up by the PAL is positioned over the MALDI target plate so that eluting droplets are spotted according to the time interval set in the PAL Method.
Abstract
Rapid and accurate identification and quantification of proteins is one of the goals of today's proteomics research. One key requirement for this approach is the ability to resolve the individual components of
peptide mixtures prior to MS analysis. The challenge is to achieve high sensitivity with limited sample amount. This requires equipment that is specifically designed for separation at low flow rates and that allows
the collection of small fraction volumes, even on MALDI targets.
The micro collection/spotting system PAL MALDI Option meets this challenge. It is an ideal tool for single or multidimensional chromatographic separation of complex peptide and protein mixtures for subsequent analysis by MALDI and/or ESI mass spectrometry. The offline combination of LC separation with MALDI MS analysis gives scientists significant advantages over the widespread online ESI/MS approach. LC/MALDI “freezes” the LC separation on the MALDI plate and allows MS/MS experiments without any time constrains. Intelligent decisions can now be made during analysis, allowing results-driven analysis without rerunning the sample. Decoupling the separation from the analysis gives the opportunity to optimize the separation performance and the total sample throughput.
Flexibility for LC/MALDI spotting on various target types from different vendors as well as collection into well plates (96 and 384). Probe positioning control of 0.1mm enables reproducible and robust collection of small fraction volumes from nl to the lower μl range. Matrix addition can be done either offline or online by premixing the matrix with the eluent. A Peltier cooling option prevents thermal decomposition and evaporation of the micro fractions. Intelligent hardware design guarantees lowest delay volumes down to a few nl to achieve optimal chromatographic resolution.
Specifications PAL MALDI Option Spotter
Compatible PAL instruments:
HTS PAL / HTC PAL
Fraction vessel capacity
HTS PAL 23 MTP or 11 Deepwell or 594 2ml vials (96 and 384 wells)
HTC PAL 11 MTP or 5 Deepwell or 270 2ml vials (96 and 384 wells)
(optional cooling to 4°C available)
MALDI Target Capacity
up to 46 depending on PAL instrument type and target vendor
Spotting Tip
Hydrophobic coated fused silica capillary
Spotting frequency
min. 3 seconds (20 spots per minute)
Delay volume
approx. 3μl with 1 meter PEEK Tubing ID 65μm (column in LC system)
approx. 10nl if column is installed inside MALDI Tool
(* lower delay volume requires tubing ID <65μm)
Flow Rate
20nl - 300μl
Transfer tubing kit
PEEK tubing ID 65μm / OD 1/16 inch
PEEK Nano Y-connector for 2 pcs. 360μm capillary tubing
Software control
Cycle Composer 1.5.3 or higher*
PAL Firmware 3.0 or higher
(*including example macros for Injection, Fraction Collection and Spotting)
Wetted Parts
All liquids compatible with PEEK, Fused Silica
Supported Targets
Bruker Daltonics, ABI/Sciex, Waters, Agilent
Description
The addition of the PAL MALDI Option coverts the HTC/HTS PAL autosampler into a fully automated injector and fraction collector/ spotter. Collect onto any surface, including MALDI Targets and micro plates.
Key Features
- Single device for sample injection and collection to MALDI plates
- Fraction collection onto MALDI or micro plates
- Re-inject collected fractions
- Use as injector for Nano LC as well
- All common MALDI targets supported
- Time based fraction collection
- Optional On-line matrix addition
Photos
System Overview |
MALDI Flow Path |
 MALDI Y Connector |
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PDF Summary of MALDI Spotter with PAL
LEAP can design MALDI holders for a variety of other vendors MALDI plates
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
Contact LEAP
 Contact LEAP
For additional information about LEAP and the PAL Platform, please contact LEAP Technologies. |
