M-001 Dual Channel Serial Injection - Analyst with ICC-CE
From LEAP
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* LEAP Shell Software and application for custom injections and scheduling [http://www.leaptec.com/software/leap-shell-3.php] | * LEAP Shell Software and application for custom injections and scheduling [http://www.leaptec.com/software/leap-shell-3.php] | ||
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[[Image:LEAP Logo.png|frame|www.leapwiki.com]] | [[Image:LEAP Logo.png|frame|www.leapwiki.com]] | ||
+ | === Contact LEAP === | ||
For additional information about this technique please contact LEAP Technologies for detailed information | For additional information about this technique please contact LEAP Technologies for detailed information |
Revision as of 16:48, 28 April 2009
LC Column Switching & Regeneration | |
Application Type | |
SPECIAL | |
Application ID | |
M-001 | |
Description | |
LC Column Switching & Regeneration. |
Contents |
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve for sample loading and a 10 port valve for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.
Key Benefits |
Saving of Reagent time (Back cut) |
Increase throughput at little cost |
Control is all within Analyst Software |
Compliance not affected |
GLP Compliant |
Restrictions |
Cannot do "Look Ahead" |
Injections are not staggered |
Peaks of interest must come off early |
Columns may perform slightly different on alternating samples |
PRINT Function for Page
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [3]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [4]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [5]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [6]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [7]
- LEAP Shell Software and application for custom injections and scheduling [8]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
Keyword: Analyst Software, ICC-CE, PAL, 10 port valve, selector valve, injector valve, gradient pump, regeneration pump,back cut, peaks of interest, GLP