M-002 Dual Channel Serial Injection with front cut - Analyst with ICC-CE
From LEAP
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a TrioVS Valve [3]. All of the valves must be controlled by the Standard LEAP cycle.
LEAP can not control the MS Selector valve as part of the cycle.
Key Benefits |
Saving of Reagent time (Back cut) [4] |
Increase throughput at little cost |
Control is all within Analyst Software |
Compliance not affected |
Avoids contaminating MS inlet with early eluting compounds and solvent |
Restrictions |
Cannot do "Look Ahead"[5] |
Injections are not staggered[6] |
Peaks of interest must come off early |
Columns may perform slightly different on alternating samples |
Requires TrioVS valve |
Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
---|---|---|---|---|---|---|
M-002 | Dual Channel Serial Injection with Front Cut Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste | Analyst with ICC-CE | * Trio VS * Gradient Pump * Regeneration pump | * Saving of regeneration time(Back Cut) * Increase throughput at little cost * Control is all within Analyst * Compliance is not affected * Avoids contaminating MS inlet with early eluting compounds and Solvent | * Can not do "Look Ahead" * Injections are not staggered * Peaks of interest must come of early * Columns may perform slightly differently on alternating samples | Yes |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [7]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [8]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [9]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [10]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [11]
- LEAP Shell Software and application for custom injections and scheduling [12]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information