H1073b – Dual Channel sample filtration

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General Description of process:

Samples are injected and pass through a guard column which is in-line with the analytical column. At a specified time, the guard column switches out of line and is regenerated using a wash pump configured to do a step gradient while the sample peaks are acquired.

To increase throughput, samples are injected ahead of the acquisition time, so that pre-filtration is occurring concurrently with the previous samples acquisition. Each line of the sample list processes a pair of samples which are staggered in this way (see time chart at the end of this document).

4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.