Dual Channel Sample Cleanup

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(New page: General Description of process: Samples are injected and pass through a guard column which is in-line with the analytical column. At a specified time, the guard column switches out of lin...)
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4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.
4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.
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'''Project Ref: H1073b'''

Revision as of 13:52, 25 March 2009

General Description of process:

Samples are injected and pass through a guard column which is in-line with the analytical column. At a specified time, the guard column switches out of line and is regenerated using a wash pump configured to do a step gradient while the sample peaks are acquired.

To increase throughput, samples are injected ahead of the acquisition time, so that pre-filtration is occurring concurrently with the previous samples acquisition. Each line of the sample list processes a pair of samples which are staggered in this way (see time chart at the end of this document).

4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.


Project Ref: H1073b

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