Hydrogen Deuterium Exchange

From LEAP

	Hydrogen Deuterium Exchange
	Application Type
	Sample Prep and Inject
	Application ID
	HD-x PAL
	Description
	Scheduling and Sample Prep for HD-x

Overview

H/D-X PAL™ is an easy-to-use, system that provides an automated process for the scheduling and experimental execution of H/D-X experimental workflow. By use of the advanced LEAP Shell scheduling software experimental design is simplified and reliable. Synchronous reagent addition and sample labeling steps are automatically scheduled to increase throughput and produce high quality data.

The application

Information on protein structure can be determined by one of 3 commonly used techniques:  Crystalography  NMR  HD Exchange

Hydrogen Deuterium exchange is an experimental technique to obtain structural data on proteins. The technique relies on the accurate measurement of the degree of labeling of a protein by deuterated hydrogen during a precisely measured labeling interval. The labeling reaction is stopped by addition of a quenching reagent.