LEAP Glossary

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LEAP Glossary of Terms
Application Type
  Glossary
Application ID
  _
Description
  Work in progress Glossary of Terms.

Contents

Overview

This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].

There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve for sample loading and a 10 port valve for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.

Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system
Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system





Key Benefits
Saving of Reagent time (Back cut)
Increase throughput at little cost
Control is all within Analyst Software
Compliance not affected
GLP Compliant



Restrictions
Cannot do "Look Ahead"
Injections are not staggered
Peaks of interest must come off early
Columns may perform slightly different on alternating samples


Image:Table M-001.png

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For other cost/time saving measures consider:

  • LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [3]
  • Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [4]
  • Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [5]
  • “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [6]
  • Dual Channel Parallel Injection (M-005) using LEAP Shell software [7]
  • LEAP Shell Software and application for custom injections and scheduling [8]
www.leapwiki.com
www.leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information



Keyword: Analyst Software, ICC-CE, PAL, 10 port valve, selector valve, injector valve, gradient pump, regeneration pump,back cut, peaks of interest, GLP

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