Balance PAL

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(New page: {{LSApp |name = Balance PAL |image = PlantExtract1.png |type = Sample Prep and Inject |id = Balance PAL |description = System for integrating a Balan...)
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=== Overview ===
=== Overview ===
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Plant material and biomass in general are not just for food and feed any longer. They have become the feedstock for fuel, neutraceuticals, drugs and food supplements such as OMEGA-3 or other strong antioxidants. There is a growing research community that looks from a genetics and maximum yield perspective at many bio-organism. A primary example are seeds for oil or algae for OMEGA-3. All these examples have one complication in common: Before analysis for the presence of certain chemicals (molecules) an extract has to be generated out of the raw sample. Fibers, skin and other natural tissue has to be filtered and many constituants of the plants that are of interest have to be extracted in an extraction and separation process. There are many procedures that use a manual approach. As long as the methodology of plant research is its trial and error stage automation can't be justified. However, once a systematic work flow is well defined an automated process is not only more productive but leads to more consistency and less wasteful use of materials, disposables, solvents and of course highly educated personnel's time.
 
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LEAP had many customers that successfully got their extractions automated. Some started out with Seeds, Algae, Tobacco and other leafy plants. The automation was in many cases "just-in time" sample preparation on LEAP's primary XYZ platform the CTC PAL with a subsequent injection onto a Gas Chromatograph or Mass Spectrometer.
 
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Often a GC Twin PAL accomplishes the extraction and filtration before a GC analyzes plant extract material. The application is to take a vial containing plant material, add solvent, heat & agitator, filter, and then analyze on a 6890 GC.  This application may be applicable to any customer who wants to do filtration providing the liquid can be pushed thru the filter plate with a low pressure inert gas through the headspace syringe.
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* Simple tedious weighing repetitions
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* Tare weight, fill weight, solvent volume calculation in spreadsheet
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* Reagent additions and accurate dilutions
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* Monitoring of weight change reactions (out gassing,digestions)
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* Vial transport (various vial sizes)
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* Two-way communication Balance-PAL
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* Most cost effective; low price tag
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* Control it with LEAP Shell <br/>
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Additional accessories such as an integrated balance (Mettler) and a barcode scanner[http://leapwiki.com/mediawiki/index.php?title=Bar_Code_Reading] have been added in the past.
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Combines liquid transfers with gravimetric determinations
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* Adaptable time schedule
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'''Markets Served:'''<br>
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* Adaptable to various vial sizes (2, 5, 10 or 20ml vials)
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Elements of this application can be applied to labs requiring any type of filtration of a sample while maximizing throughput.
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* Results from weighing recorded in LEAP Shell software
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* University
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* Use automatic bar code scanner
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* Research
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* Small footprint
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* FDA
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* High vial capacity
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* EPA
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* Works with capped vials and reagent reservoirs
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* USDA
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* Add reagent holder and inkwell bottles
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* Cooling option to keep samples stable or heating for faster reaction
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'''What is a Plant Extract experiment?'''<br/>
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The sample prep procedure is quite simple with the exception of the final sample needing to not contain any of the plant material when it gets to the analysis procedure. The samples are incubated in a heated agitator, and then allowed to settle.  The sample solvent, possible containing small pieces of plant material, is injected onto a 96 well filter plate in the top of a SPE drawer with a 19ga headspace syringe using the Combi PAL, the needle guide is locked, and then flushed (purged) with a stream of inert gas (e.g. Helium) forcing the solvent through the filter plate into the receiving plate below. The other PAL then makes the liquid injection from the receiving plate into the GC.
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'''LEAPS Approach'''<br/>
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The GC Twin PAL in combination with the cycle composer makes a very effective and flexible workstation. We were able to design and provide a solution which met the following challenges from the customer:<br/>
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# Provide the necessary filtration to have clean samples to analyze. In order to get good blow-out of the liquid through the filter, it was determined that the filter plate should be sealed and have a small nozzle on the underside to ensure accurate collection without crossover to adjacent collection vials.<br/>
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# Throughput. By using a Twin PAL we are able to take advantage of having one PAL do the preparation while the other does the sample injections.<br/>
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# Spatial concerns. Provide a way to get everything that was required to fit. <br/>
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=== Photos ===
=== Photos ===

Revision as of 01:44, 29 May 2009

Balance PAL
Application Type
  Sample Prep and Inject
Application ID
  Balance PAL
Description
  System for integrating a Balance with the PAL
leaptec.comleapwiki.com
leaptec.com
leapwiki.com

Contents

[hide]

Overview

  • Simple tedious weighing repetitions
  • Tare weight, fill weight, solvent volume calculation in spreadsheet
  • Reagent additions and accurate dilutions
  • Monitoring of weight change reactions (out gassing,digestions)
  • Vial transport (various vial sizes)
  • Two-way communication Balance-PAL
  • Most cost effective; low price tag
  • Control it with LEAP Shell


Combines liquid transfers with gravimetric determinations

  • Adaptable time schedule
  • Adaptable to various vial sizes (2, 5, 10 or 20ml vials)
  • Results from weighing recorded in LEAP Shell software
  • Use automatic bar code scanner
  • Small footprint
  • High vial capacity
  • Works with capped vials and reagent reservoirs
  • Add reagent holder and inkwell bottles
  • Cooling option to keep samples stable or heating for faster reaction

Photos

Automation of Plant Extract before Gas Chromatography

Seed Crusher

Other Reference Material

A high-throughput screen for genes from castor that boost

Hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis
leaptec.comleapwiki.com
leaptec.com
leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information

Retrieved from "http://leapwiki.com/mediawiki/index.php/Balance_PAL"
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