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Revision as of 20:31, 28 April 2009 (view source) Bpeat (Talk | contribs) (New page: {{LSApp |name = LC Column Switching & Regeneration |image = 2_valve_on_top.png |type = '''SPECIAL''' |id = M-001 |description = LC Column Switching...) ← Older edit		Revision as of 20:32, 28 April 2009 (view source) Bpeat (Talk | contribs) Newer edit →
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-	[[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]]
-	=== Overview ===
-	This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within '''Analyst Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc'' using a Standard LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php].
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-	There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/6-port-2-position-valve-155.php] for sample loading and a 10 port valve [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/10-port-2-position-valve-157.php]for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.
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-	[[Image:M-001.png|frame|Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system]]
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Revision as of 20:32, 28 April 2009

	LC Column Switching & Regeneration
	Application Type
	SPECIAL
	Application ID
	M-001
	Description
	LC Column Switching & Regeneration.

Key Benefits

Saving of Reagent time (Back cut)

Increase throughput at little cost

Control is all within Analyst Software

Compliance not affected

GLP Compliant

Restrictions

Cannot do "Look Ahead"

Injections are not staggered

Peaks of interest must come off early

Columns may perform slightly different on alternating samples

Quick Overview
Code	Application	Control SW	Key Hardware	Key Benefits	Restrictions	GLP
M-001	Column switching with parallel regeneration Provides shorter cycle times by regenerating columns during next analytical run	Analyst with ICC-CE	PAL 6 port injection valve 10 port Valve Gradient Pump and Regeneration pump	Saving of regeneration time(Back Cut) Increase throughput at little cost Control is all within Analyst Compliance is not affected	Can not do "Look Ahead" Injections are not staggered Peaks of interest must come of early Columns may perform slightly differently on alternating samples	Yes

PRINT Function for Page

• PDF Summary of New System

• PDF Summary of Upgrade to Existing System

For other cost/time saving measures consider:

• LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [1]
• Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [2]
• Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [3]
• “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [4]
• Dual Channel Parallel Injection (M-005) using LEAP Shell software [5]
• LEAP Shell Software and application for custom injections and scheduling [6]

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Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information

• Contact the LEAP office