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| LC Column Switching & Regeneration |
| Application Type | |
| SPECIAL | |
| Application ID | |
| M-001 | |
| Description | |
| LC Column Switching & Regeneration. |
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Contents |
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[3] for sample loading and a 10 port valve [4]for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.
| Key Benefits |
| Saving of Reagent time (Back cut) |
| Increase throughput at little cost |
| Control is all within Analyst Software |
| Compliance not affected |
| GLP Compliant |
| Restrictions |
| Cannot do "Look Ahead" |
| Injections are not staggered |
| Peaks of interest must come off early |
| Columns may perform slightly different on alternating samples |
| Quick Overview | ||||||
| Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
|---|---|---|---|---|---|---|
| M-001 | Column switching with parallel regeneration Provides shorter cycle times by regenerating columns during next analytical run | Analyst with ICC-CE | PAL 6 port injection valve 10 port Valve Gradient Pump and Regeneration pump | Saving of regeneration time(Back Cut) Increase throughput at little cost Control is all within Analyst Compliance is not affected | Can not do "Look Ahead" Injections are not staggered Peaks of interest must come of early Columns may perform slightly differently on alternating samples | Yes |
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For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [5]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [6]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [7]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [8]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [9]
- LEAP Shell Software and application for custom injections and scheduling [10]
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Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
