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| - | === Overview ===
| + | '''Sorry for any inconvenience ''' |
| - | This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within '''Analyst Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc'' using a Standard LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php].
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| - | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve for sample loading and a 10 port valve for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.
| + | Please see Glossary at http://www.leapwiki.com/mediawiki/index.php?title=Glossary |
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| - | [[Image:M-001.png|frame|Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system]]
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| - | <br><br><br><br>
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| - | {|
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| - | | '''[[Key Benefits]]'''
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| - | | '''Saving of Reagent time (Back cut)'''
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| - | | '''Increase throughput at little cost'''
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| - | | '''Control is all within Analyst Software'''
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| - | | '''Compliance not affected'''
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| - | | '''GLP Compliant'''
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| - | |}
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| - | <br><br>
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| - | | '''[[Restrictions]]'''
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| - | | Cannot do "Look Ahead"
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| - | | Injections are not staggered
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| - | | Peaks of interest must come off early
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| - | | Columns may perform slightly different on alternating samples
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| - | |}
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| - | [[Image:Table M-001.png]]
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| - | === PRINT Function for Page ===
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| - | *[[:Image:M001_Dual_Column_with_Regeneration_Estimate_20090401.pdf | PDF Summary of New System]]
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| - | *[[:Image:Upgrade__M001_Dual_Column_with_Regeneration_Estimate_20090402.pdf | PDF Summary of Upgrade to Existing System]]
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| - | === For other cost/time saving measures consider:===
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| - | * LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing]
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| - | * Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-002_Dual_Channel_Serial_Injection_with_front_cut_-_Analyst_with_ICC-CE]
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| - | * Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [http://www.leapwiki.com/mediawiki/index.php?title=M-003_Dual_Channel_Parallel_Injection_-_Cycle_Composer]
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| - | * “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [http://www.leaptec.com/software/leap-shell-3.php]
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| - | * Dual Channel Parallel Injection (M-005) using LEAP Shell software [http://www.leapwiki.com/mediawiki/index.php?title=M-005_Dual_Channel_Parallel_Injection_-_LEAP_Shell_%22(M-Fab)%22]
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| - | * LEAP Shell Software and application for custom injections and scheduling [http://www.leaptec.com/software/leap-shell-3.php]
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| - | [[Image:LEAP Logo.png|frame|www.leapwiki.com]]
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| - | === Contact LEAP ===
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| - | For additional information about this technique please contact LEAP Technologies for detailed information
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| - | * [http://www.leaptec.com/contact-us.php Contact the LEAP office]
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| - | Keyword: Analyst Software, ICC-CE, PAL, 10 port valve, selector valve, injector valve, gradient pump, regeneration pump,back cut, peaks of interest, GLP
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