M-002 Dual Channel Serial Injection with front cut - Analyst with ICC-CE
From LEAP
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This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within '''Analyst Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc'' using a Standard LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php]. | This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within '''Analyst Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc'' using a Standard LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php]. | ||
- | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a TrioVS Valve [http://www.leaptec.com/products/lc-related-instruments-and-accessories/rotors-and-inlet-sleeves/pal-trio-vs--valve-system--80.php]. All of the valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle. | + | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a TrioVS Valve [http://www.leaptec.com/products/lc-related-instruments-and-accessories/rotors-and-inlet-sleeves/pal-trio-vs--valve-system--80.php]. All of the valves must be controlled by the Standard LEAP cycle. <br> ''LEAP can not control the MS Selector valve as part of the cycle.'' |
[[Image:Empty TRIO.png|frame|TrioVS valve is used. Flow path intentionally left incomplete. Contact LEAP for more information]] | [[Image:Empty TRIO.png|frame|TrioVS valve is used. Flow path intentionally left incomplete. Contact LEAP for more information]] |
Revision as of 18:19, 11 May 2009
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a TrioVS Valve [3]. All of the valves must be controlled by the Standard LEAP cycle.
LEAP can not control the MS Selector valve as part of the cycle.
Key Benefits |
Saving of Reagent time (Back cut) [4] |
Increase throughput at little cost |
Control is all within Analyst Software |
Compliance not affected |
Avoids contaminating MS inlet with early eluting compounds and solvent |
Restrictions |
Cannot do "Look Ahead"[5] |
Injections are not staggered[6] |
Peaks of interest must come off early |
Columns may perform slightly different on alternating samples |
Requires TrioVS valve |
Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
---|---|---|---|---|---|---|
M-002 | Dual Channel Serial Injection with Front Cut Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste | Analyst with ICC-CE | * Trio VS * Gradient Pump * Regeneration pump | * Saving of regeneration time(Back Cut) * Increase throughput at little cost * Control is all within Analyst * Compliance is not affected * Avoids contaminating MS inlet with early eluting compounds and Solvent | * Can not do "Look Ahead" * Injections are not staggered * Peaks of interest must come of early * Columns may perform slightly differently on alternating samples | Yes |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [7]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [8]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [9]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [10]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [11]
- LEAP Shell Software and application for custom injections and scheduling [12]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information