Dual Channel Sample Cleanup

From LEAP

(Difference between revisions)
Jump to: navigation, search
Line 5: Line 5:
To increase throughput, samples are injected ahead of the acquisition time, so that pre-filtration is occurring concurrently with the previous samples acquisition. Each line of the sample list processes a pair of samples which are staggered in this way (see time chart at the end of this document).
To increase throughput, samples are injected ahead of the acquisition time, so that pre-filtration is occurring concurrently with the previous samples acquisition. Each line of the sample list processes a pair of samples which are staggered in this way (see time chart at the end of this document).
-
4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.
+
4 pumps are required: - 2 wash pumps (Agilent 1200) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.
Line 14: Line 14:
'''Project Ref: H1073b'''
'''Project Ref: H1073b'''
 +
 +
-----
 +
[[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]]
=== Contact LEAP ===
=== Contact LEAP ===
 +
 +
For additional information about this technique please contact LEAP Technologies for detailed information
* [http://www.leaptec.com/contact-us.php Contact the LEAP office]
* [http://www.leaptec.com/contact-us.php Contact the LEAP office]
 +
 +
 +
<br><br>
 +
[[Category:LEAP Shell]]

Revision as of 02:18, 29 April 2009

General Description of process:

Samples are injected and pass through a guard column which is in-line with the analytical column. At a specified time, the guard column switches out of line and is regenerated using a wash pump configured to do a step gradient while the sample peaks are acquired.

To increase throughput, samples are injected ahead of the acquisition time, so that pre-filtration is occurring concurrently with the previous samples acquisition. Each line of the sample list processes a pair of samples which are staggered in this way (see time chart at the end of this document).

4 pumps are required: - 2 wash pumps (Agilent 1200) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.


Image:Dual_Channel_Sample_Filtration_PlumbDiag.png


Project Ref: H1073b



www.leaptec.comwww.leapwiki.com
www.leaptec.com
www.leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information




Personal tools