Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery
From LEAP
Journal of Pharmaceutical and Biomedical Analysis 34 (2004)1-9 Edward H. Kerns a,, Teresa Kleintop a, David Little b, Thomas Tobien c, Larry Mallis d,LiDia, Mei Hu a, Yan Hong a, Oliver J. McConnell d a Wyeth Research, CN8000, Princeton, NJ 08543-8000, USA b Micromass, Manchester, UK c LEAP Technologies, Carrboro, NC, USA d Wyeth Research, Collegeville, PA, USA Received 16 September 2002; received in revised form 20 March 2003; accepted 1May2003
Abstract A method is described for use in analysis of samples from pharmaceutical profiling of early drug discovery compounds. The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns operated in parallel for alternating trapping, washing and elution into a tandem quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) MS/MS mode. A primary method, which is useful for 80-90% of compounds, and a secondary method, which is useful for a majority of the remaining compounds, are described. No analytical HPLC column is used and the analysis rate is approximately 50 samples/h. Specificity is obtained using MRM analysis. Application of the method for high capacity analysis of metabolic stability samples is described.
Full article available upon request
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LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics’ PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
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